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Series GSE4286 Query DataSets for GSE4286
Status Public on Oct 05, 2007
Title Making a predictive heart failure expression profile in Ren2 rat left ventricles
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The intercalated disc of cardiac myocytes is emerging as a crucial structure in the heart. Loss of intercalated disc proteins like N-cadherin causes lethal cardiac abnormalities, mutations in intercalated disc proteins cause human cardiomyopathy. A comprehensive screen for novel mechanisms in failing hearts demonstrated that expression of the lysosomal integral membrane protein-2 (LIMP-2) is increased in cardiac hypertrophy and heart failure in both rat and human myocardium. Complete loss of LIMP-2 in genetically engineered mice did not affect cardiac development; however these LIMP-2 null mice failed to mount a hypertrophic response to increased blood pressure but developed cardiomyopathy. Disturbed cadherin localization in these hearts suggested that LIMP-2 has important functions outside lysosomes. Indeed, we also find LIMP-2 in the intercalated disc, where it associates with cadherin. RNAi-mediated knockdown of LIMP-2 decreases the binding of phosphorylated b-catenin to cadherin, while overexpression of LIMP-2 has the opposite effect. Taken together, our data show that lysosomal integrated membrane protein-2 is crucial to mount the adaptive hypertrophic response to cardiac loading. We demonstrate a novel role for LIMP-2 as an important mediator of the intercalated disc.
Keywords: heart failure, comparison
Overall design overall design:
3 groups of rats, 1 sample per rat:
- compensated = Ren2 rat, hypertensive, no heart failure (N=6)
- failure = Ren2 rat, hypertensive, no heart failure (N=4)
- SD = control group, non-hypertensive (N=4)
Contributor(s) van Erk A, Schroen B, Pinto Y
Citation(s) 17485520
Submission date Feb 21, 2006
Last update date Jul 31, 2017
Contact name Arie van Erk
Phone +31 (043) 3882913
Organization name University Maastricht
Department BiGCaT Bioinformatics
Lab Experimental and Molecular Cardiology
Street address PO Box 616
City Maastricht
ZIP/Postal code 6200MD
Country Netherlands
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (14)
GSM178755 Ren2, compensated, rat 1
GSM180057 Ren2, compensated, rat 2
GSM180059 Ren2, compensated, rat 3
BioProject PRJNA94779

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4286_RAW.tar 174.8 Mb (http)(custom) TAR (of CEL, CHP, EXP)
Processed data included within Sample table
Processed data provided as supplementary file

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