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Series GSE42938 Query DataSets for GSE42938
Status Public on Aug 15, 2013
Title Abrupt scrib- vs. Abrupt and RasV12 (RasACT) scrib-, NotchICD (NACT) scrib- +/- JNK (Bsk) expression profiles
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Drosophila mosaic eye-antennal discs from the listed genotypes generated using the MARCM system were dissected from 3rd instar larvae at day 5 after egg deposition.
20 pairs of discs for the Abrupt and scrib- + Abrupt samples and 50 pairs from FRT control, NACT scrib- +/- BskDN and RasACT scrib- +/- BskDN were used to prepare RNA. Samples were prepared in triplicate, and the RNA isolated using TRIZOL, before being column purified (Qiagen). Probes were hybridized to GeneChip Drosophila 2.0 Genome Arrays (Affymetrix).
Overall design To compare the expression profile of Abrupt when overexpressed in the eye-antennal discs with tumours formed by Abrupt overexpression in scrib- clones, and to reveal JNK responsive genes in RasV12 (RasACT) scrib- versus NotchICD (NACT) scrib- eye-antennal mosaic discs
Contributor(s) Richardson HE, Ellul J
Citation(s) 23874226, 26207831
Submission date Dec 14, 2012
Last update date Nov 06, 2019
Contact name Helena Elizabeth Richardson
Phone 61396561466
Organization name Peter MacCallum Cancer Center
Department Research Division
Lab Cell Cycle and Development lab
Street address 7 St Andrew's place
City East Melbourne
State/province Victoria
ZIP/Postal code 3002
Country Australia
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (21)
GSM1053590 mosaic larval eye-antennal discs Abrupt overexpressing 1
GSM1053591 mosaic larval eye-antennal discs Abrupt overexpressing 2
GSM1053592 mosaic larval eye-antennal discs Abrupt overexpressing 3
BioProject PRJNA183895

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42938_RAW.tar 40.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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