We used methylation DNA arrays to observe the genomic wild methylation profiles in 31 malignant ovarian tumors. After validations, we selected, NEFH and HS3ST2. We used bisulfite pyrosequencing to expose methylation status for 84 independent samples. Kaplan–Meier survival and Cox regression analysis estimates the association with ovarian cancer outcome. We discovered patients with high methyatlion of two genes, had a shorter median 5-years overall survival. We also evaluated the high-methylation of a panel genes in patients, that presented as an independat factor for associated with poor outcomes. High-methylation of NEFH and HS3ST2 are provided the prognostic index for the poor outcome of overall survival in ovarian cancers.
Overall design
Bisulphite converted DNA from the 31 samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2
Less...
More...