|
| Status |
Public on Aug 28, 2013 |
| Title |
Heterokaryon RNA Sequencing |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the application of bi-species RNAseq for investigating mechanisms of reprogramming towards pluripotency using heterokaryons (mouse embryonic stem cell X human fibroblast cell fusions). The use of mixed species allows one to monitor reprogramming of the human somatic nuclei independently of contributions from the mouse nuclei using nucleotide differences. We used RNAseq to monitor heterokaryon reprogramming over a 3-day timecourse, generating transcriptome-wide data for cell fusion based reprogramming of human fibroblasts towards pluripotency.
|
| |
|
| Overall design |
Examination of cellular reprogramming over a heterokaryon timecourse (mouse embryonic stem cells X human fibroblasts)
|
| |
|
| Contributor(s) |
Brady JJ, Blau HM |
| Citation(s) |
23995732 |
| |
| Submission date |
Jan 16, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Jennifer Brady |
| Organization name |
Stanford University
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL16512 |
Illumina HiSeq 2000 (Homo sapiens; Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA186683 |
| SRA |
SRP017972 |
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