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Series GSE43965 Query DataSets for GSE43965
Status Public on Feb 01, 2013
Title Studies of target genes for signal transduction pathways (using a Ras1-, a Cdc42- and a Gap1-mutant) in the basidiomycete Schizophyllum commune.
Organism Schizophyllum commune
Experiment type Expression profiling by array
Summary Transcriptome changes associated with signal transduction mutants were analysed in order to identify targets of the distinct pathways of Ras1, Cdc42 and Gap1. Two monokaryons (12-43 and 4-39), a dikaryon (W22 x 12-43), a constitutive active Ras1 mutant (T2G12V, monokaryon), a Cdc42 mutant (G12V, monokaryon) and a ∆gap1 x ∆gap1 dikaryon (F15 x F28, deletion of Gap1) were analyzed for that purpose.
 
Overall design With microarray analysis mRNA expression in different mutants of the basidiomycete S. commune was analyzed. Genes which show regulation in the comparison either of the two monokaryons 12-43 and 4-39 (for Ras2 and Cdc42 mutant) or the dikaryon W22 x 12-43 (for Gap1 x Gap1 dikaryon) have been eliminated from other comparisons as stage-specific (mono- or dikaryon) differences. Two biological replicate samples were analyzed for each condition.
 
Contributor(s) Knabe N, Freihorst D, Erdmann S, Kothe E
Citation(s) 23606288
Submission date Jan 31, 2013
Last update date May 14, 2013
Contact name Erika Kothe
E-mail(s) erika.kothe@uni-jena.de
Organization name Friedrich-Schiller-University
Department Microbial Communication, Institute of Microbiology
Street address Neugasse 25
City Jena
ZIP/Postal code 07743
Country Germany
 
Platforms (1)
GPL11376 FSU-Schco1-Microarray v1.0
Samples (6)
GSM1075072 T2G12V_constitutive-active-Ras1_biological_replicate_1
GSM1075073 T2G12V_constitutive-active-Ras1_biological_replicate_2
GSM1075074 cdc42_G12V_mutant_biological_replicate_1
Relations
BioProject PRJNA188239

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43965_raw_data.txt.gz 272.4 Kb (ftp)(http) TXT
Processed data included within Sample table

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