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Status |
Public on Mar 08, 2013 |
Title |
The Role of Tet1 During Somatic Cell Reprogramming |
Organism |
Mus musculus |
Experiment type |
Methylation profiling by high throughput sequencing Expression profiling by high throughput sequencing
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Summary |
Tet1 is a hydroxylase known for its role in the conversion of 5-methylcytosines (5mC) to 5-hydroxymethylcytosines (5hmC) involved in the possible active demethylation process and gene expression regulation. As somatic cell reprogramming involves the re-activation of pluripotency genes and the silencing of somatic ones, it remains unclear the role of Tet1 in the reprogramming process. Here, we performed hMeDIP-seq and RNA-seq during somatic cells reprogramming with Tet1 over expression to invest the effect of Tet1.
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Overall design |
Examination of genome DNA hmC modifications in 2 conditions: individually overexpressed Tet1CD or Tet2CD during MEF reprogramming; Examination of mRNA levels in five different conditions: individually overexpressed DR or Tet1CD or Tet1CDmut or Tet2CD or Tet2CDmut, during MEF reprogrammig.
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Contributor(s) |
Pei D, Xu G |
Citation(s) |
24162740 |
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Submission date |
Mar 06, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Lihui Lin |
E-mail(s) |
lin_lihui@gibh.ac.cn
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Phone |
020-32015291
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Organization name |
Guangzhou Institutes Of Biomedicine and Health Chinese Academy Of Science
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Department |
Stem cell
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Lab |
Pei Duanqing
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Street address |
No.190, Kaiyuan Street
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City |
Guangzhou |
State/province |
Guangdong |
ZIP/Postal code |
GD20 |
Country |
China |
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Platforms (2) |
GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
GPL16417 |
Illumina MiSeq (Mus musculus) |
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Samples (7)
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Relations |
BioProject |
PRJNA192583 |
SRA |
SRP018969 |