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Series GSE45248 Query DataSets for GSE45248
Status Public on Mar 18, 2013
Title Defining a molecular roadmap of cellular reprogramming into iPS Cells [DNA methylation]
Organism Mus musculus
Experiment type Methylation profiling by genome tiling array
Summary Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is inefficient, complicating mechanistic studies. Here, we examined defined intermediate cell populations poised to becoming iPSCs by genome-wide analyses. We show that induced pluripotency elicits two transcriptional waves, which are driven by c-Myc/Klf4 (first wave) and Oct4/Sox2/Klf4 (second wave). Cells that become refractory to reprogramming activate the first but fail to initiate the second transcriptional wave and can be rescued by elevated expression of all four factors. The establishment of bivalent domains occurs gradually after the first wave, whereas changes in DNA methylation take place after the second wave when cells acquire stable pluripotency. This integrative analysis allowed us to identify genes that act as roadblocks during reprogramming and surface markers that further enrich for cells prone to forming iPSCs. Collectively, our data offer new mechanistic insights into the nature and sequence of molecular events inherent to cellular reprogramming.
 
Overall design DNA methylation profiles obtained at different timepoints during reprogramming of somatic cells: Day 0, +3, +6, +9 and iPSC
 
Contributor(s) Polo J, Figueroa ME, Hochedlinger K
Citation(s) 23260147
Submission date Mar 18, 2013
Last update date Mar 18, 2013
Contact name Ben S. Wittner
E-mail(s) wittner.ben@mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Center for Cancer Research
Lab Lawrence
Street address 149 13th Street
City Boston
State/province MA
ZIP/Postal code 02129
Country USA
 
Platforms (1)
GPL10283 Nimblegen Mouse 25K MM5_HELP_Promoter array
Samples (5)
GSM1099769 Day_0
GSM1099770 Day_3
GSM1099771 Day_6
This SubSeries is part of SuperSeries:
GSE42478 Defining a molecular roadmap of cellular reprogramming into iPS cells
Relations
BioProject PRJNA193330

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45248_RAW.tar 83.1 Mb (http)(custom) TAR (of PAIR)
Processed data included within Sample table

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