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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 14, 2014 |
| Title |
Escalating-dose peptide immunotherapy induces progressive immunoregulatory changes in self-reactive T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Peptide immunotherapy aims to specifically restore tolerance to the administered self-antigen and prevent autoimmunity without the perturbation of normal immune function. We have developed a dose escalation protocol for subcutaneous delivery of the MHC II-restricted myelin basic protein peptide analogue Ac1-9[4Y] to T cell receptor transgenic (Tg4) mice. Dose escalation allows safe administration of high doses of peptide, which effectively induces antigen-specific tolerance and suppresses the development of experimental autoimmune encephalomyelitis, a model for the human condition multiple sclerosis. CD4+ T cells isolated from treated mice are anergic and suppressive in vitro and respond to stimulation by secretion of the immunoregulatory cytokine IL-10. To understand the molecular changes occurring throughout the course of dose-escalation immunotherapy, we undertook microarray analysis of CD4+ T cells at different the stages of treatment, using Tg4 Rag-1 deficient mice, which lack naturally occurring regulatory T cells and have a monoclonal CD4+ T cell population
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| Overall design |
CD4+ T cells were collected from Tg4 Rag1 deficient TCR-transgenic mice at six sequential stages of EDI plus a PBS-treated control, RNA extracted and then subjected to microarray hybridization using Affymetrix Mouse Gene 1.0 ST array
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| Contributor(s) |
Burton BR, Britton GJ, Fang H, Gough JJ, Wraith DC |
| Citation(s) |
25182274 |
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| Submission date |
Apr 12, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Hai Fang |
| E-mail(s) |
hfang@cs.bris.ac.uk
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| Organization name |
University of Bristol
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| Department |
Department of Computer Science
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| Street address |
Woodland Road
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| City |
Bristol |
| ZIP/Postal code |
BS8 1UB |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (7)
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| GSM1122169 |
3 treatment using 0.08ug, 0.8ug, 8ug peptide prime |
| GSM1122170 |
4 treatment using 0.08ug, 0.8ug, 8ug, 80ug peptide prime |
| GSM1122171 |
6 treatment using 0.08ug, 0.8ug, 8ug, 80ug x 3 peptide prime |
| GSM1122172 |
10 treatment using 0.08ug, 0.8ug, 8ug, 80ug x 7 peptide prime |
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| Relations |
| BioProject |
PRJNA196942 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE46036_RAW.tar |
31.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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