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Series GSE46314 Query DataSets for GSE46314
Status Public on Apr 24, 2013
Title Development of gene expression signatures to characterize evolution of hASC cells isolated and maintained xenogeneic-free
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human adipose stem cells (hASCs) play a crucial role in the fields of regenerative medicine and tissue engineering for different reasons: the abundance of adipose tissue, their easy harvesting, the ability to multipotent differentiation and the fact that they do not trigger allogeneic blood response or secrete cytokines that act as immunosuppressants. The vast majority of protocols use animal origin reagents, with the underlying risk of transmitting infections by non-human pathogens. We have designed a protocol to isolate and maintain the properties of hASCs avoiding xenogeneic reagents. These changes not only preserve hASCs morphology, but also increase cell proliferation and maintain their stem cell marker profile. On the other hand, human serum albumin (HSA), Tryple® and human Serum (HS), do not affect hASCs multipotent differentiation ability. The amendments introduced do not trigger modifications in the transcriptional profile of hASCs, alterations in key biochemical pathways or malignization. Thus, we have proven that it is possible to isolate and maintain hASCs avoiding animal reagents and, at the same time, preserving crucial culture parameters during long term culture. Thereby we have revealed a novel and effective tool for the improvement of clinical, cell-based therapies.
 
Overall design Gene expression in human adipose stem cells isolated and maintained with human reagents were measured immediately after their isolation, and at passages 1, 3 and 5. Pools from the same 8 healthy donors were used to measure the 4 points of growth.
 
Contributor(s) Carmen E, Andres S
Citation(s) 23874459
Submission date Apr 23, 2013
Last update date Feb 22, 2018
Contact name Andrés Sanz-García
E-mail(s) sanz.andres@gmail.com
Organization name University of La Rioja
Lab EDMANS
Street address C/ Luis de Ulloa 20
City Logroño
State/province La Rioja
ZIP/Postal code 26001
Country Spain
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (4)
GSM1128570 hASC_after_isolation_Rep1
GSM1128571 hASC_Passage1_Rep1
GSM1128572 hASC_Passage3_Rep1
Relations
BioProject PRJNA198722

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46314_RAW.tar 43.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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