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Series GSE46423 Query DataSets for GSE46423
Status Public on Apr 27, 2013
Title Minotaur is critical for primary piRNA biogenesis [RNA-Seq]
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Piwi proteins and their associated small RNAs are essential for fertility in animals. This is due, in part, to their roles in guarding germ cell genomes against the activity of mobile genetic elements. piRNA populations direct Piwi proteins to silence transposon targets and as such form a molecular code that discriminates transposons from endogenous genes. Information ultimately carried by piRNAs is encoded within genomic loci, termed piRNA clusters. These give rise to long, single-stranded, primary transcripts that are processed into piRNAs. Despite the biological importance of this pathway, neither the characteristics that define a locus as a source of piRNAs nor the mechanisms that catalyze primary piRNA biogenesis are well understood. We searched an EMS-mutant collection annotated for fertility phenotypes for genes involved in the piRNA pathway. Twenty-seven homozygous-sterile strains showed transposon-silencing defects. One of these, which strongly impacted primary piRNA biogenesis, harbored a causal mutation in CG5508, a member of the Drosophila glycerol-3-phosphate O-acetyltransferase (GPAT) family. These enzymes catalyze the first acylation step on the path to the production of phosphatidic acid (PA). Though this pointed strongly to a function for phospholipid signaling in the piRNA pathway, a mutant form of CG5508, which lacks the GPAT active site, still functions in piRNA biogenesis. We have named this new biogenesis factor Minotaur.
Overall design Examination of transcriptom profile in heterozygous and homozygous CG5508 mutant ovaries
Contributor(s) Vagin VV, Yu Y, Jankowska A, Luo Y, Wasik K, Malone CD, Harrison E, Rosebrock A, Wakimoto BT, Fagegaltier D, Muerdter F, Hannon GJ
Citation(s) 23788724
Submission date Apr 26, 2013
Last update date May 15, 2019
Contact name Vasily Vagin
Organization name Cold Spring Harbor
Lab Hannon's
Street address 1 Bungtown Rd
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
Platforms (1)
GPL9061 Illumina Genome Analyzer II (Drosophila melanogaster)
Samples (2)
GSM1129995 het_CG5508_ovaries_RNAseq
GSM1129996 hom_CG5508_ovaries_RNAseq
This SubSeries is part of SuperSeries:
GSE46424 Minotaur is critical for primary piRNA biogenesis
BioProject PRJNA200307
SRA SRP021535

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Supplementary file Size Download File type/resource
GSE46423_uniq_mappers_downregulated_in_homCG5508.txt.gz 1.7 Kb (ftp)(http) TXT
GSE46423_uniq_mappers_upregulated_in_homCG5508.txt.gz 4.4 Kb (ftp)(http) TXT
GSE46423_uniq_piRNAcluster_mappers_upregulated_in_homCG5508.txt.gz 691 b (ftp)(http) TXT
GSE46423_up_to_10mappers_downregulated_in_homCG5508.txt.gz 1.5 Kb (ftp)(http) TXT
GSE46423_up_to_10mappers_upregulated_in_homCG5508.txt.gz 15.2 Kb (ftp)(http) TXT
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