|
| Status |
Public on Apr 27, 2013 |
| Title |
The Presomitic Mesoderm (PSM) CREB family transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Dynamic gene expression in the PSM of vertebrates is critical for the spatial and temporal patterning of somites.
Using microarray analysis, we explored in detail, genes that are differentially regulated upon removal of CREB family function from the mouse PSM.
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| |
|
| Overall design |
Mouse PSM from R26R^AC/AC (Control) and R26R^AC/AC;T-Cre (Mutant) were harvested for RNA extraction. Nine PSM's from control and mutant genotypes were combined to generate three biological replicates (three PSM's/replicate). Samples were then applied to microarray analysis to identify differentially expressed genes between controls and mutants.
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| |
|
| Contributor(s) |
Lopez TP, Fan C |
| Citation(s) |
23671110 |
| |
| Submission date |
Apr 26, 2013 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Timothy Peter Lopez |
| E-mail(s) |
tpetelopez@gmail.com
|
| Organization name |
Johns Hopkins University/Carnegie Institution
|
| Department |
Biology
|
| Lab |
Chen-Ming Fan
|
| Street address |
3520 San Martin Drive
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21218 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (6)
|
| GSM1130053 |
R26R^AC/AC (Control), biological replicate #1 |
| GSM1130054 |
R26R^AC/AC (Control), biological replicate #2 |
| GSM1130055 |
R26R^AC/AC (Control), biological replicate #3 |
| GSM1130056 |
R26R^AC/AC;T-Cre (Mutant), biological replicate #1 |
| GSM1130057 |
R26R^AC/AC;T-Cre (Mutant), biological replicate #2 |
| GSM1130058 |
R26R^AC/AC;T-Cre (Mutant), biological replicate #3 |
|
| Relations |
| BioProject |
PRJNA200312 |
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