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Series GSE46507 Query DataSets for GSE46507
Status Public on May 01, 2013
Title Comparison of the nucleotide-resolution, genome-wide, 5'-end maps of the transcriptomes of Escherichia coli K12 strain BW25113 and Streptomyces coelicolor A3(2) strain M145
Organisms Streptomyces coelicolor A3(2); Escherichia coli BW25113
Experiment type Expression profiling by high throughput sequencing
Summary The differential RNA-seq data contained within this entry is complemented by global RNA-seq.
 
Overall design S. coelicolor and E. coli reference strains were grown in batch culture with shaking. The E. coli sample was extracted during mid-exponential growth, while the S. coelicolor sample was extracted at the start of pigmented antibiotic production. Each of the two samples was incubated with or without TAP (tobacco acid pyrophosphatase) before library construction. Thus, 4 libraries were analysed. TAP treatment allows the cloning and sequencing of 5' ends that were originally triphosphorylated.
 
Contributor(s) Romero DA, Lin Y, McDowall KJ
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Submission date Apr 30, 2013
Last update date May 15, 2019
Contact name David Romero A.
E-mail(s) efy4dr@leeds.ac.uk
Organization name University of Leeds
Street address Woodhouse lane
City Leeds
State/province West Yorkshire
ZIP/Postal code LS29JT
Country United Kingdom
 
Platforms (2)
GPL15206 Illumina HiSeq 2000 (Escherichia coli BW25113)
GPL17096 Illumina HiSeq 2000 (Streptomyces coelicolor A3(2))
Samples (4)
GSM1131348 E. coli - TAP
GSM1131349 E. coli + TAP
GSM1131350 S. coelicolor - TAP
Relations
BioProject PRJNA200704
SRA SRP021913

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46507_RAW.tar 4.3 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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