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Status |
Public on May 01, 2013 |
Title |
Comparison of the nucleotide-resolution, genome-wide, 5'-end maps of the transcriptomes of Escherichia coli K12 strain BW25113 and Streptomyces coelicolor A3(2) strain M145 |
Organisms |
Streptomyces coelicolor A3(2); Escherichia coli BW25113 |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The differential RNA-seq data contained within this entry is complemented by global RNA-seq.
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Overall design |
S. coelicolor and E. coli reference strains were grown in batch culture with shaking. The E. coli sample was extracted during mid-exponential growth, while the S. coelicolor sample was extracted at the start of pigmented antibiotic production. Each of the two samples was incubated with or without TAP (tobacco acid pyrophosphatase) before library construction. Thus, 4 libraries were analysed. TAP treatment allows the cloning and sequencing of 5' ends that were originally triphosphorylated.
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Contributor(s) |
Romero DA, Lin Y, McDowall KJ |
Citation missing |
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Submission date |
Apr 30, 2013 |
Last update date |
May 15, 2019 |
Contact name |
David Romero A. |
E-mail(s) |
efy4dr@leeds.ac.uk
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Organization name |
University of Leeds
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Street address |
Woodhouse lane
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City |
Leeds |
State/province |
West Yorkshire |
ZIP/Postal code |
LS29JT |
Country |
United Kingdom |
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Platforms (2) |
GPL15206 |
Illumina HiSeq 2000 (Escherichia coli BW25113) |
GPL17096 |
Illumina HiSeq 2000 (Streptomyces coelicolor A3(2)) |
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Samples (4)
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Relations |
BioProject |
PRJNA200704 |
SRA |
SRP021913 |