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| Status |
Public on Apr 14, 2006 |
| Title |
24 hour time course: regulation of uterine genes by estradiol in ovariectomized mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Using microarray technology, we compared the global expression pattern of uterine RNA from ovariectomized control mice to those of ovariectomized mice treated with estradiol for various intervals between 30 minutes and 24 hours.
Keywords: estrogen, uterus, genomic, mouse
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| Overall design |
Mice (C57 BI/6) were treated with sesame oil or estradiol and uteri were collected at 30 minutes, two hours, 6 hours, 12 hours and 24 hours after treatment and snap frozen in liquid nitrogen. Three or four uteri from each treatment group were pooled and RNA was prepared using Trizol reagent and the RNeasy clean up protocol. Two arrays for each time point were used, incorporating a fluor reversal.
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| Contributor(s) |
Korach KS, Hewitt SC, Deroo BJ, Grissom SF, Collins JB |
| Citation(s) |
12893882 |
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| Submission date |
Apr 13, 2006 |
| Last update date |
Dec 06, 2012 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
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| Organization name |
NIEHS
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| Department |
DIR
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| Lab |
Microarray Core
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| Street address |
111 T.W. Alexander Drive
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| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platforms (1) |
| GPL891 |
Agilent-011978 Mouse Microarray G4121A (Feature Number version) |
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| Samples (10)
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| GSM105232 |
time course control vs. E, 30 min (ExpID 2702) |
| GSM105233 |
E, 30 min vs. time course control (ExpID 2702) |
| GSM105234 |
time course control vs. E, 2 hr (ExpID 2703) |
| GSM105235 |
E, 2 hr vs. time course control (ExpID 2703) |
| GSM105236 |
time course control vs. E, 6 hr (ExpID 2704) |
| GSM105237 |
E, 6 hr vs. time course control (ExpID 2704) |
| GSM105238 |
time course control vs. E, 12 hr (ExpID 2705) |
| GSM105239 |
E, 12 hr vs. time course control (ExpID 2705) |
| GSM105240 |
time course control vs. E, 24 hr (ExpID 2706) |
| GSM105241 |
E, 24 hr vs. time course control (ExpID 2706) |
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| Relations |
| BioProject |
PRJNA96809 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE4664_RAW.tar |
307.6 Mb |
(http)(custom) |
TAR (of TIFF, TXT) |
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