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| Status |
Public on Jan 20, 2022 |
| Title |
Expression profiling of Ciona intestinalis initial tailbud stage embryos expressing mutant forms of the transcription factor Ci-XBP1 in the notochord. |
| Organism |
Ciona intestinalis |
| Experiment type |
Expression profiling by array
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| Summary |
XBP transcription factors are well-known regulators of the the unfolded protein response and are required for plasma cell differentiation. However, recent studies have shown that the XBP1 gene is also expressed in the developing notochord of Ciona and various vertebrates, and to date its role in the formation of this organ is largely uncharacterized. We identified putative targets of Ci-XBP1 through a microarray screen, using RNAs extracted from embryos expressing mutant forms of this transcriptional regulator in the notochord.
We expressed in the Ciona notochord a dominant-negative version of Ci-XBP1 (XBP DBD::GFP) by cloning its DNA-binding domain (DBD) downstream of the Ci-Brachyury (Ci-Bra) promoter. We created a constitutive activator form of Ci-XBP by fusing its DBD to the VP16 transactivation domain (Bra>XBP DBD::VP16::GFP). These constructs were introduced into 1-cell stage embryos and grown to the initial tailbud (iTb) stage to identify Ci-XBP notochord target genes.
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| Overall design |
Genes expressed in embryos carrying the XBP DBD::GFP and XBP DBD::VP16::GFP were compared genes expressed in wild-type controls (Bra>GFP) at the initial tailbud using whole-genome custom Affymetrix microarrays.
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| Contributor(s) |
Di Gregorio A |
| Citation(s) |
35049502 |
| |
| Submission date |
May 08, 2013 |
| Last update date |
Feb 08, 2022 |
| Contact name |
Anna Di Gregorio |
| E-mail(s) |
adg13@nyu.edu
|
| Phone |
212-998-9564
|
| Organization name |
NYU College of Dentistry
|
| Department |
Basic Science and Craniofacial Biology
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| Lab |
Di Gregorio
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| Street address |
345 E 24th Street, room 804A
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10010 |
| Country |
USA |
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| Platforms (1) |
| GPL15657 |
[CINT06a520380F] Affymetrix Ciona intestinalis custom-designed array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA202244 |
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