GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE46751 Query DataSets for GSE46751
Status Public on Jan 20, 2022
Title Expression profiling of Ciona intestinalis initial tailbud stage embryos expressing mutant forms of the transcription factor Ci-XBP1 in the notochord.
Organism Ciona intestinalis
Experiment type Expression profiling by array
Summary XBP transcription factors are well-known regulators of the the unfolded protein response and are required for plasma cell differentiation. However, recent studies have shown that the XBP1 gene is also expressed in the developing notochord of Ciona and various vertebrates, and to date its role in the formation of this organ is largely uncharacterized. We identified putative targets of Ci-XBP1 through a microarray screen, using RNAs extracted from embryos expressing mutant forms of this transcriptional regulator in the notochord.
We expressed in the Ciona notochord a dominant-negative version of Ci-XBP1 (XBP DBD::GFP) by cloning its DNA-binding domain (DBD) downstream of the Ci-Brachyury (Ci-Bra) promoter. We created a constitutive activator form of Ci-XBP by fusing its DBD to the VP16 transactivation domain (Bra>XBP DBD::VP16::GFP). These constructs were introduced into 1-cell stage embryos and grown to the initial tailbud (iTb) stage to identify Ci-XBP notochord target genes.
Overall design Genes expressed in embryos carrying the XBP DBD::GFP and XBP DBD::VP16::GFP were compared genes expressed in wild-type controls (Bra>GFP) at the initial tailbud using whole-genome custom Affymetrix microarrays.
Contributor(s) Di Gregorio A
Citation(s) 35049502
Submission date May 08, 2013
Last update date Feb 08, 2022
Contact name Anna Di Gregorio
Phone 212-998-9564
Organization name NYU College of Dentistry
Department Basic Science and Craniofacial Biology
Lab Di Gregorio
Street address 345 E 24th Street, room 804A
City New York
State/province NY
ZIP/Postal code 10010
Country USA
Platforms (1)
GPL15657 [CINT06a520380F] Affymetrix Ciona intestinalis custom-designed array
Samples (9)
GSM1138154 control, replicate 1
GSM1138155 control, replicate 2
GSM1138156 control, replicate 3
BioProject PRJNA202244

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46751_RAW.tar 42.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap