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| Status |
Public on Sep 13, 2015 |
| Title |
Murine ES Cells: Control vs. ETV4/5 double KO |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
ETS-related transcription factors ETV4 and ETV5 play crucial roles for organogenesis and morphogenesis. We compared the transcriptional profiles between wild-type and ETV4 and ETV5 double knockout (ETV4/5 dKO) ES cells by an oligo DNA microarray analysis. Self-renewal capacity and pluripotency are known to be controlled by an ES cell-specific transcription factor network; therefore, we focused on transcription-associated genes. Of 1258 transcription-related genes, 47 genes were significantly downregulated and 98 genes were significantly upregulated in ETV4/5 dKO ES cells. Several genes whose expression is specific to undifferentiated ES were repressed in ETV4/5 dKO ES cells. In contrast, expression of differentiation markers was enhanced in ETV4/5 dKO ES cells.
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| Overall design |
Transcriptional profiles between wild-type and ETV4/5 dKO ES cells were examined by an oligo DNA microarray analysis. ES cells were cultured on gelatin-coated dishes with LIF-supplemented ES cell medium. Total RNAs were isolated from wild-type and ETV4/5 dKO ES cells and subjected to microarray analysis.
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| Contributor(s) |
Akagi T, Yokota T |
| Citation(s) |
26224636 |
| |
| Submission date |
May 23, 2013 |
| Last update date |
Apr 19, 2018 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
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| Platforms (1) |
| GPL5642 |
3D-Gene Mouse Oligo chip 24k |
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| Samples (1) |
| GSM1147068 |
Murine ES Cells: Control vs. ETV4/5 double KO |
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| Relations |
| BioProject |
PRJNA205239 |
