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Series GSE47225 Query DataSets for GSE47225
Status Public on Sep 13, 2015
Title Murine ES Cells: Control vs. ETV4/5 double KO
Organism Mus musculus
Experiment type Expression profiling by array
Summary ETS-related transcription factors ETV4 and ETV5 play crucial roles for organogenesis and morphogenesis. We compared the transcriptional profiles between wild-type and ETV4 and ETV5 double knockout (ETV4/5 dKO) ES cells by an oligo DNA microarray analysis. Self-renewal capacity and pluripotency are known to be controlled by an ES cell-specific transcription factor network; therefore, we focused on transcription-associated genes. Of 1258 transcription-related genes, 47 genes were significantly downregulated and 98 genes were significantly upregulated in ETV4/5 dKO ES cells. Several genes whose expression is specific to undifferentiated ES were repressed in ETV4/5 dKO ES cells. In contrast, expression of differentiation markers was enhanced in ETV4/5 dKO ES cells.
Overall design Transcriptional profiles between wild-type and ETV4/5 dKO ES cells were examined by an oligo DNA microarray analysis. ES cells were cultured on gelatin-coated dishes with LIF-supplemented ES cell medium. Total RNAs were isolated from wild-type and ETV4/5 dKO ES cells and subjected to microarray analysis.
Contributor(s) Akagi T, Yokota T
Citation(s) 26224636
Submission date May 23, 2013
Last update date Apr 19, 2018
Contact name Satoshi Kondo
Organization name Toray Industries,Inc.
Department New Projects Development Division
Street address Tebiro 6-10-1
City Kamakura
State/province Kanagawa
ZIP/Postal code 248-8555
Country Japan
Platforms (1)
GPL5642 3D-Gene Mouse Oligo chip 24k
Samples (1)
GSM1147068 Murine ES Cells: Control vs. ETV4/5 double KO
BioProject PRJNA205239

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Supplementary file Size Download File type/resource
GSE47225_RAW.tar 2.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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