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Series GSE4768 Query DataSets for GSE4768
Status Public on May 06, 2006
Title Global analysis of transcriptional changes following genomic demethylation in chondrocytes, osteoblasts, and fibroblasts
Organism Mus musculus
Experiment type Expression profiling by array
Methylation profiling by array
Summary DNA methylation can contribute to the stable transcriptional silencing of mammalian genes. Oftentimes, these genes are important developmental regulators, and their silencing in cell types where they are not supposed to be active is important for the phenotypic stability of the cells. To identify key developmental regulator genes whose expression in terminally differentiated cells may be inhibited by DNA methylation, mouse dermal fibroblasts were demethylated with 5-aza-2’-deoxycytidine, and changes in gene expression monitored by microarray analysis.
Keywords: 5-aza-2'-deoxycytidine; methylation; dermal fibroblast; chondrocyte; osteoblast
Overall design Three biological replicates for both control and 5-aza-2'-deoxycytidine treatment were derived. Cells were primary mouse dermal fibroblasts, primary mouse chondrocytes, and primary mouse osteoblasts derived separately for each biological replicate. Treated cells were exposed to 5uM 5-aza-2'-deoxycytidine for 96 hours with recovery in normal medium for 24 hours. Control cells were untreated.
Contributor(s) Vallender TF
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Submission date May 03, 2006
Last update date Feb 11, 2019
Contact name Tammy Vallender
Phone 7738344065
Organization name University of Chicago
Department Human Genetics
Lab Dr. Bruce Lahn
Street address 929 E 57th St W503V
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (18)
GSM106151 fibroblast_5azatreated_rep1
GSM106152 fibroblast_5azacontrol_rep1
GSM106153 fibroblast_5azatreated_rep2
BioProject PRJNA95649

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