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Series GSE48561 Query DataSets for GSE48561
Status Public on Nov 07, 2013
Title High-resolution genome replication profiles, modeling and single-cell imaging define the stochastic nature of replication initiation and termination
Organism Saccharomyces cerevisiae
Experiment type Other
Summary Eukaryotic genome replication is stochastic with each cell using a different cohort of replication origins. Interpreting high-resolution genome replication profiles with a mathematical model allowed us to quantify the stochastic nature of genome replication. This approach included estimation of the activity of every replication origin and the genome-wide location of replication termination events. Single-cell measurements verified the inferred values for stochastic origin replication time. Strains in which multiple origins had been inactivated, confirmed that the location of termination events is primarily dictated by the stochastic activation time of origins. Cell-to-cell variability in origin activity ensures that termination events are widely distributed across virtually the whole genome. We propose that the heterogeneity in origin usage contributes to genome stability by limiting potentially deleterious events from accumulating at particular loci.
Overall design Measurement of genome replication time for two S. cerevisiae strains. For each strain six S phase samples werecompared with a non-replicating sample.
Contributor(s) Nieduszynski CA, Müller CA, Hawkins M, Retkute R
Citation(s) 24210825
Submission date Jul 05, 2013
Last update date May 15, 2019
Contact name Conrad A Nieduszynski
Organization name Earlham Institute
Lab DNA replication & genome stability
Street address Norwich Research Park
City Norwich
ZIP/Postal code NR4 7UZ
Country United Kingdom
Platforms (1)
GPL16234 AB 5500 Genetic Analyzer (Saccharomyces cerevisiae)
Samples (16)
GSM1180942 wt_alpha
GSM1180943 wt_cdc7
GSM1180944 wt_15min
BioProject PRJNA210660
SRA SRP026571

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Supplementary file Size Download File type/resource
GSE48561_RAW.tar 430.0 Kb (http)(custom) TAR (of WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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