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| Status |
Public on Jul 16, 2013 |
| Title |
Murine Schistosoma-Induced Pulmonary Hypertension: Microarray Data |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Rationale: Schistosomiasis is one of the most common causes of pulmonary arterial hypertension worldwide, but the pathogenic mechanism by which the host inflammatory response contributes to vascular remodeling is unknown. We sought to identify signaling pathways that play protective or pathogenic roles in experimental Schistosoma-induced pulmonary vascular disease by whole-lung transcriptome analysis. Methods: Wildtype mice were experimentally exposed to S. mansoni ova by intraperitoneal sensitization followed by tail vein augmentation, and the phenotype assessed by right ventricular catheterization and tissue histology, RNA and protein analysis. Whole-lung transcriptome analysis by microarray and RNA sequencing was performed, the latter analyzed using 2 bioinformatic methods. Functional testing of the candidate IL-6 pathway was determined using IL6-knockout mice and the STAT3 inhibitor STI-201. Results: Wild-type mice exposed to S. mansoni had increased right ventricular systolic pressure and thickness of the pulmonary vascular media. Whole lung transcriptome analysis identified the IL6-STAT3-NFATc2 pathway as being upregulated, which was confirmed by PCR and immunostaining of lung tissue from S. mansoni-exposed mice and patients who died of the disease. Mice lacking IL6 or treated with STI-201 developed pulmonary hypertension associated with significant intima remodeling after exposure to S. mansoni. Conclusions: Whole lung transcriptome analysis identified upregulation of the IL6-STAT3-NFATc2 pathway, and IL6 signaling was found to be protective against Schistosoma-induced intimal remodeling.
Affy Mouse ST1.0 chip used. Data published in: Protective Role of IL6 in Vascular Remodeling in Schistosoma-Pulmonary Hypertension. Graham BB, Chabon J, Kumar R, Kolosionek E, Gebreab L, Debella E, Edwards M, Diener K, Shade T, Bifeng G, Bandeira A, Butrous G, Jones K, Geraci M, Tuder RM. Am J Respir Cell Mol Biol. 2013 Jul 1. [Epub ahead of print] PMID: 23815102
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| Overall design |
Whole lung transcriptome of 3 mice with experimental Schistosoma-induced pulmonary hypertension, compared to 3 control mice. All mice on a C57Bl6/J background.
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| Contributor(s) |
Graham BB, Chabon J |
| Citation(s) |
23815102, 25621148 |
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| Submission date |
Jul 16, 2013 |
| Last update date |
Aug 16, 2019 |
| Contact name |
Brian Graham |
| E-mail(s) |
brian.graham@ucdenver.edu
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| Organization name |
University of Colorado Denver
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| Department |
Medicine
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| Street address |
12700 E 19th Ave, C-272
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| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE49116 |
Protective Role of IL6 in Vascular Remodeling in Schistosoma-Pulmonary Hypertension |
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| Relations |
| BioProject |
PRJNA212332 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48936_RAW.tar |
22.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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