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Status |
Public on Jul 22, 2013 |
Title |
The role of Dichaete in transcriptional regulation of Drosophila embryogenesis |
Organism |
Drosophila melanogaster |
Experiment type |
Expression profiling by array
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Summary |
Dichaete is a developmentally important transcription factor, known to be involved in basic biological processes including segmentation and nervous system development among others. The aim of this experiment was to gain further insight into the role of Dichaete during early embryogenesis, by looking at the disruption of gene expression in Dichaete mutants.
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Overall design |
Stage 10-11 embryos (5 and 7.5 hours after egg laying) from a cross between Dr72/TM3, twi-GAL4 UAS-Gfp Dr513/TM3, twi-GAL4 UAS-Gfp, were hand picked under a fluorescence dissecting microscope. GFP negative homozygous Dichaete mutant embryos and their heterozygous single GFP positive siblings were collected and approximately 150 embryos per sample were stored frozen in Trizol
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Contributor(s) |
Aleksic J, Fischer B, Russell S |
Citation missing |
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Submission date |
Jul 22, 2013 |
Last update date |
Dec 10, 2013 |
Contact name |
Steve Russell |
E-mail(s) |
sr120@hermes.cam.ac.uk
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Organization name |
University of Cambridge
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Department |
Genetics
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Street address |
Tennis Court Road
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City |
Cambridge |
ZIP/Postal code |
CB2 3EH |
Country |
United Kingdom |
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Platforms (1) |
GPL14121 |
FL003 - INDAC Drosophila melanogaster 14.5K long oligo array (FlyBase r.5.39) |
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Samples (4)
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GSM1193278 |
D homozygous mutant vs heterozygous control rep 1 |
GSM1193279 |
D homozygous mutant vs heterozygous control rep 2 |
GSM1193280 |
D homozygous mutant vs heterozygous control rep 3 |
GSM1193281 |
D homozygous mutant vs heterozygous control rep 4 |
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This SubSeries is part of SuperSeries: |
GSE49095 |
The role of Dichaete in transcriptional regulation during Drosophila embryonic development |
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Relations |
BioProject |
PRJNA212993 |