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| Status |
Public on Oct 30, 2014 |
| Title |
Identifying Targets of DSX with ChIP-seq |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We have used ChIP-seq on Drosophila S2 cells to characterize genome-wide DSX occupancy.
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| Overall design |
We have perfomed ChIP-seq on S2 cells transfected with either V5-tagged DSXF or DSXM constructs. We performed three biological replicates for each ChIP sample and two biological replicates for the input samples. For peak calling and analysis, the reads from all biological replicates were pooled before aligning them to the genome.
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| Contributor(s) |
Clough E, Jimenez E, Kim Y, Whitworth C, Neville M, Hempel L, Pavlou H, Chen Z, Sturgill D, Dale R, Smith HE, Przytycka TM, Goodwin S, Van Doren M, Oliver B |
| Citation(s) |
25535918 |
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| Submission date |
Aug 02, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Brian Oliver |
| E-mail(s) |
briano@nih.gov
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| Phone |
301-204-9463
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| Organization name |
NIDDK, NIH
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| Department |
LBG
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| Lab |
Developmental Genomics
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| Street address |
50 South Drive
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL9058 |
Illumina Genome Analyzer (Drosophila melanogaster) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE49480 |
Identifying targets of DSX with ChIP-seq, DamID-seq and DamID-chip and transcriptional response to DSX isoform switch |
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| Relations |
| BioProject |
PRJNA214088 |
| SRA |
SRP028410 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49511_RAW.tar |
35.7 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
| GSE49511_S2_DsxF.peaks.1.11.bed.gz |
54.9 Kb |
(ftp)(http) |
BED |
| GSE49511_S2_DsxM.peaks.1.11.bed.gz |
45.4 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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