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Series GSE49532 Query DataSets for GSE49532
Status Public on Dec 23, 2013
Title The C. elegans CSR-1 argonaute pathway counteracts epigenetic silencing to promote germline gene expression
Organism Caenorhabditis elegans
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Organisms can develop adaptive sequence-specific immunity by reexpressing pathogen-specific small RNAs that guide gene silencing. For example, the C. elegans PIWI-Argonaute/piwi-interacting RNA (piRNA) pathway recruits RNA-dependent RNA polymerase (RdRP) to foreign sequences to amplify a transgenerational small-RNA-induced epigenetic silencing signal (termed RNAe). Here, we provide evidence that, in addition to an adaptive memory of silenced sequences, C. elegans can also develop an opposing adaptive memory of expressed/self-mRNAs. We refer to this mechanism, which can prevent or reverse RNAe, as RNA-induced epigenetic gene activation (RNAa). We show that CSR-1, which engages RdRP-amplified small RNAs complementary to germline-expressed mRNAs, is required for RNAa. We show that a transgene with RNAa activity also exhibits accumulation of cognate CSR-1 small RNAs. Our findings suggest that C. elegans adaptively acquires and maintains a transgenerational CSR-1 memory that recognizes and protects self-mRNAs, allowing piRNAs to recognize foreign sequences innately, without the need for prior exposure.
 
Overall design Examine small RNA population changes in different transgene lines
 
Contributor(s) Seth M, Shirayama M, Gu W, Ishidate T, Conte D, Mello C
Citation(s) 24360782
Submission date Aug 04, 2013
Last update date May 15, 2019
Contact name Craig Mello
Organization name University of Massachusetts Medical School
Department Program in Molecular Medicine
Lab Craig Mello
Street address 368 Plantatoin Street, Suite AS5-2047
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
 
Platforms (1)
GPL13657 Illumina HiSeq 2000 (Caenorhabditis elegans)
Samples (7)
GSM1201393 0320 total oma-1::gfp
GSM1201394 0320 total oma-1::gfp rde-3
GSM1201395 0320 total oma-1::gfp csr-1
Relations
BioProject PRJNA214176
SRA SRP028513

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49532_RAW.tar 102.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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