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| Status |
Public on Aug 14, 2013 |
| Title |
HuR and miR-1192 regulate myogenesis by modulating the translation of HMGB1 mRNA (mRNA) |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Upon muscle injury the high mobility group box 1 (HMGB1) protein is up-regulated and secreted to initiate reparative responses. Here we show that HMGB1 controls myogenesis both in vitro and in vivo, during development and after adult muscle injury. HMGB1 expression in muscle cells is regulated at the translational level: the miRNA miR-1192 inhibits HMGB1 translation and the RNA-binding protein HuR promotes it. HuR binds to a cis-element, HuRBS, located in the 3'UTR of the HMGB1 transcript, and at the same time miR-1192 is recruited to an adjacent seed element. The binding of HuR to the HuRBS prevents the recruitment of Argonaute 2 (Ago2), overriding miR-1192-mediated translation inhibition. Depleting HuR reduces myoblast fusion and silencing miR-1192 re-establishes the fusion potential of HuR-depleted cells. We propose that HuR promotes the commitment of myoblasts to myogenesis by enhancing the translation of HMGB1 and suppressing the translation inhibition mediated by miR-1192.
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| Overall design |
RNA content was extracted following immunoprecipitation of HuR using a monoclonal antibody (3A2) and the levels of mRNA were compared to an IgG control in order to determine which transcripts were enriched in the HuR ribonucleoprotein complex.
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| Contributor(s) |
Dormoy-Raclet V, Cammas A, Celona B, Lian X, van der Giessen K, Zivojnovic M, Brunelli S, Riuzzi F, Sorci G, Wilhelm BT, Di Marco S, Donato R, Bianchi ME, Gallouzi I |
| Citation(s) |
24005720 |
| |
| Submission date |
Aug 13, 2013 |
| Last update date |
Nov 13, 2013 |
| Contact name |
Imed Gallouzi |
| E-mail(s) |
imed.gallouzi@mcgill.ca
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| Phone |
514-398-4537
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| Organization name |
McGill University
|
| Department |
Biochemistry
|
| Lab |
915
|
| Street address |
3655 Promenade Sir William Osler
|
| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H3G1Y6 |
| Country |
Canada |
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| Platforms (1) |
| GPL17568 |
miRCURY™ LNA microRNA array v11.0 hmr, miRBase 13.0 + miRPlus |
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| Samples (2) |
| GSM1208294 |
RNA from Immunoprecipitation with IgG Antibody |
| GSM1208295 |
RNA from Immunoprecipitation with HuR Antibody |
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| This SubSeries is part of SuperSeries: |
| GSE49857 |
HuR and miR-1192 regulate myogenesis by modulating the translation of HMGB1 mRNA |
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| Relations |
| BioProject |
PRJNA215097 |
