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Series GSE49920 Query DataSets for GSE49920
Status Public on Aug 15, 2013
Title Antenatal maternal long-term hypoxia: acclimatization responses with altered gene expression in ovine fetal carotid arteries
Organism Ovis aries
Experiment type Expression profiling by array
Summary In humans and other species, Longlong-term hypoxia (LTH) during pregnancy can lead to intrauterine growth restriction with reduced body/brain weight, dysregulation of cerebral blood flow (CBF), and other problems in humans and rodents. In contrast, sheep appear to undergo relatively successful acclimatization, not demonstrating any of the above-mentioned problems except at extremely high altitude. To identify the signal transduction genetic pathways and those critical molecules, which may be involved in acclimatization to high altitude LTH, we conducted microarray with advanced bioinformatic analysis on carotid arteries (CA) from the normoxic near-term ovine fetus at sea-level and those acclimatized to high altitude for 110+ days during gestation. In response to LTH acclimatization, in fetal CA we identified, mRNA from 38 genes upregulated (> 2 fFold; (P < 0.05) and 9 genes downregulated (> 2-f Fold; (P < 0.05). The major genes with upregulated mRNA were SLC1A3, Insulin-like growth factor (IGF) binding protein 3, IGF type 2 receptor, transforming growth factor (TGF) Beta-3, and genes involved in the AKT and BCL2 signal transduction networks. The majority of genes with upregulated mRNA have a common motif for Pbx/Knotted homeobox in the promoter region, and Sox family binding sites in the 3’ un -translated region (UTR). Genes with downregulated mRNA included those involved in the P53 pathway and 5-lipoxygenase activating proteins. The promoter region of all genes with downregulated mRNA, had a common 49 bp region, with a binding site for DOT6 and TOD6, components of the RPD3 histone deacetylase complex RPD3C(L). We also identified miRNA complementary to a number of the altered genes. Thus, the present study identified molecules in the ovine fetus, which may help it to play a role in the acclimatizatione successfully response to high-altitude associated LTH.
 
Overall design In these series of experiments we examined changes in gene expression in sheep carotids. Pregnant sheep and non-pregnant adult sheep were exposed to 110 days of hypoxia at 3801 meters of altitude. Carotid arteries from fetuses from non-pregnant adult from sea-level controls and those from high-altitude were compared by Agilent ovine custom array
 
Contributor(s) Goyal R
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Submission date Aug 15, 2013
Last update date Aug 15, 2013
Contact name Ravi Goyal
E-mail(s) rgoyal@live.com
Phone 9092531596
Organization name University of Arizona
Street address PO Box 64682
City TUCSON
State/province Arizona
ZIP/Postal code 85728
Country USA
 
Platforms (1)
GPL10778 Agilent-019921 Sheep Gene Expression Microarray (Probe Name version)
Samples (8)
GSM1209682 Fetus sea level control 1
GSM1209683 Fetus sea level control 2
GSM1209684 Fetus sea level control 3
Relations
BioProject PRJNA215330

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49920_RAW.tar 17.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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