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Series GSE50243 Query DataSets for GSE50243
Status Public on Nov 26, 2013
Title FOXO3 selectively amplifies enhancer activity to establish target gene regulation.
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Forkhead Box O (FOXO) transcription factors are versatile players in diverse cellular processes, affecting tumorigenesis, metabolism, stem cell maintenance and lifespan. To understand the transcriptional output of FOXO3 activation, we investigate features that define the subset of enhancer binding events that actually contribute to gene regulation. We show FOXO3 transcriptional output is determined by the amount of bound FOXO3, which in turn is determined by motif presence, pre-existing enhancer activity and accessibility. In this manner, FOXO3 amplifies pre-existing levels of activity marks and potentiates enhancer RNA transcription. We conclude that not only enhancer presence and sequence content, but also the pre-existing activity dictates FOXO3 binding and transcriptional output. Considering the flexible and cell type specific nature of regulatory regions and their activity, our observations provide a novel explanation for the diversity in FOXO transcriptional programs and introduce chromatin context as a new player in the regulation of FOXO activity in ageing and disease.
Overall design Examination of histone modifications and transcriptome changes upon FOXO activation
Contributor(s) Mokry M
Citation(s) 24360957
Submission date Aug 27, 2013
Last update date May 15, 2019
Contact name Michal Mokry
Organization name Wilhelmina Children's Hospital, University Medical Center Utrecht
Street address Lundlaan 6
City Utrecht
ZIP/Postal code 3584 EA
Country Netherlands
Platforms (1)
GPL16288 AB 5500xl Genetic Analyzer (Homo sapiens)
Samples (18)
GSM1216735 CHIP_DL23_4OHT_4h_H3
GSM1216736 CHIP_DL23_4OHT_4h_H3K27ac
GSM1216737 CHIP_DL23_4OHT_4h_H3K4me1
BioProject PRJNA217329
SRA SRP029259

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE50243_RAW.tar 654.6 Mb (http)(custom) TAR (of BED, RPKM)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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