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Series GSE50403 Query DataSets for GSE50403
Status Public on Aug 29, 2013
Title Bisphosphonate effect on murine whole draining lymph nodes before and 8 hours after VSV infection
Organism Mus musculus
Experiment type Expression profiling by array
Summary Bisphosphonates are a class of drugs that are widely used to inhibit loss of bone mass in patients. We show that the administration of clinically relevant doses of bisphosphonates in mice increases antibody responses to live and inactive viruses, proteins, haptens and existing commercial vaccine formulations. Bisphosphonates exert this adjuvant-like activity in the absence of CD4+ and γδ T cells, neutrophils or dendritic cells and their effect does not rely on local macrophage depletion nor does it depend upon Toll-like receptor signaling or the inflammasome. Rather, bisphosphonates target directly B cells and enhance B cell expansion and antibody production upon antigen encounter. These data establish bisphosphonates as a novel class of adjuvants that boost humoral immune responses. Of note, gene-expression profile analysis shows that bisphosphonate treatment up-regulates a number of B cell-specific transcripts and gene sets associated with B cell function, suggesting that bisphosphonates might directly target B cells.
 
Overall design C57Bl/6 mice received intrafoodpad injections of Clodronate (CLD, 2mg) or vehicle control (PBS) 3 and 1 day prior to infection with VSV serotype Indiana in the same footpad. Popliteal lymph nodes were harvested immediately before or 8 hours after the infection. Total RNA was extracted and prepared for hybridization on Affymetrix microarrays.
 
Contributor(s) Tonti E, Chevier N, Guidotti LG, von Andrian UH, Iannacone M
Citation(s) 24120862
Submission date Aug 28, 2013
Last update date Aug 06, 2018
Contact name Elena Tonti
E-mail(s) etonti80@hotmail.com
Organization name San Raffaele Scientific Institute
Street address Via Olgettina 58
City Milan
ZIP/Postal code 20132
Country Italy
 
Platforms (1)
GPL11180 [HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate
Samples (8)
GSM1218183 LN_PBS_VSV0h_rep1
GSM1218184 LN_PBS_VSV0h_rep2
GSM1218185 LN_PBS_VSV8h_rep1
Relations
BioProject PRJNA217414

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50403_RAW.tar 17.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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