|
Status |
Public on Dec 31, 2014 |
Title |
Deep sequencing of small RNA libraries reveals dynamic expression patterns of miRNAs in multiple developmental stages of Bactrocera dorsalis |
Organism |
Bactrocera dorsalis |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
Summary |
Four developmental stage small RNA libraries including Eggs, Larvae, Pupae and Adults were constructed, where a lot of known miRNAs were identified and many novel miRNAs were predicted. Comparison of their expression profiles in the four libraries suggested that Bactrocera dorsalis miRNAs are dynamically regulated throughout the life cycle.Further analysis of the expression and function of these miRNAs could increase our understanding of regulatory networks in the insect and lead to novel approaches to its control.
|
|
|
Overall design |
The total RNAs from eggs, larvae, pupae and adults stages of Bactrocera dorsalis were used to construct small RNA libraries for Solexa sequencing.The data from Solexa sequencer (Illumina) was compared to identify small RNAs in different stages of Bactrocera dorsalis.
|
|
|
Contributor(s) |
Huang Y, Dou W, Wang J |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Aug 29, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Yong Huang |
E-mail(s) |
zhugezhengwu@163.com
|
Organization name |
Southwest University
|
Street address |
Tiansheng Road
|
City |
Chongqing |
ZIP/Postal code |
400716 |
Country |
China |
|
|
Platforms (1) |
GPL17657 |
Illumina Genome Analyzer (Bactrocera dorsalis) |
|
Samples (4)
|
|
Relations |
BioProject |
PRJNA217508 |
SRA |
SRP029337 |