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Series GSE505 Query DataSets for GSE505
Status Public on Jul 16, 2003
Title Serial analysis of gene expression in the corneal endothelium of Fuchs' dystrophy
Organism Homo sapiens
Experiment type Expression profiling by SAGE
Summary PURPOSE: To compare the gene expression profiles of normal human corneal endothelium with Fuchs' corneal endothelium, by using serial analysis of gene expression (SAGE). METHODS: Three pairs of normal human corneas were obtained from eye banks. Thirteen bisected Fuchs' corneal buttons were processed at the time of corneal transplantation. The endothelia of normal and Fuchs'-affected corneas were stripped, and total RNA was isolated. Serial analysis of gene expression (SAGE) was performed to identify and quantify gene transcripts. Genes over- and underexpressed by Fuchs' endothelium were limited to P < 0.01 by the method of Audic and Claverie. RESULTS: A total of 19,136 tags were identified with 9,530 from normal and 9,606 from Fuchs' endothelium. The expression of 18 transcripts was upregulated, and 36 transcripts were downregulated in Fuchs' endothelium compared with normal tissue. Upregulated transcripts included serum amyloid A1 and A2, metallothionein, and apolipoprotein D. Of the downregulated transcripts, 26 matched known genes, 3 matched expressed sequence tags (ESTs), and 7 were unknown to current databases. One downregulated transcript involved a newly reported bicarbonate transporter. Decreased transcripts related to antioxidants and proteins conferring protection against toxic stress were noted in Fuchs' versus normal endothelium including nuclear ferritin, glutathione S-transferase-pi, and heat shock 70-kDa protein. Nine different SAGE tags matching mitochondrial sequences accounted for 25% of the ESTs that were decreased in Fuchs' endothelium. CONCLUSIONS: SAGE analysis comparing normal to Fuchs' endothelium demonstrates diminished expression of mitochondrial, pump function, and antiapoptotic cell defense genes. A complete report of gene expression profiles of normal human corneal endothelium can be found in PMID 12583793.
Keywords: other
Contributor(s) Gottsch JD, Seitzman GD, Margulies EH, Bowers AL, Kim SW, Saha S, Jun AS, Stark WJ, Liu SH
Citation(s) 12556388, 12583793
Submission date Jul 02, 2003
Last update date Jul 23, 2013
Contact name Elliott H Margulies
Phone 301-594-9210
Organization name National Human Genome Research Institute
Department Genome Technology Branch
Lab Dr. Eric Green
Street address
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL4 SAGE:10:NlaIII:Homo sapiens
Samples (2)
GSM1652 nce
GSM1653 fce
BioProject PRJNA85221

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