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Series GSE5097 Query DataSets for GSE5097
Status Public on Jun 21, 2006
Title Genomic responses to ectopic expression of DHR96 in Drosophila melanogaster
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary DHR96 plays a role in regulating xenobiotic responses in Drosophila. Using a gain-of-function approach we test whether DHR96 is sufficient to affect detoxification genes in the absence of a xenobiotic insult.
Keywords: gain-of-function
Overall design A transgenic line that expresses a full-length DHR96 cDNA under heat-shock control was generated by standard P-element-mediated transformation, using w1118 as a host strain. Control flies are therefore w1118 without any transgene. Larvae were staged at the molt from L2 to L3, and heat-treated after 3 hours for one hour at 37.5 degrees (in vials). Finally, RNA was extracted 4 hours later.
Contributor(s) King-Jones K, Horner MA, Lam G, Thummel CS
Citation(s) 16814731
Submission date Jun 16, 2006
Last update date May 04, 2018
Contact name Kirst King-Jones
Phone 780-4928605
Organization name University of Alberta
Department Biological Sciences
Lab King-Jones
Street address 77 University Campus
City Edmonton
State/province AB
ZIP/Postal code T6H 5V3
Country Canada
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (6)
GSM115017 Ectopic expression of DHR96 (gain of function) control 1
GSM115020 Ectopic expression of DHR96 (gain of function) control 2
GSM115021 Ectopic expression of DHR96 (gain of function) control 3
BioProject PRJNA95349

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