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Series GSE510 Query DataSets for GSE510
Status Public on Jul 16, 2003
Title Sepsis gene expression profiling: murine splenic compared with hepatic responses
Organism Mus musculus
Experiment type Expression profiling by array
Summary The aim of this series of experiments was to gain experience in sepsis gene expression profiling in a well-accepted model of murine polymicrobial abdominal sepsis and begin characterizing (in the parlance of genomics) the sepsis "transcriptome." DESIGN: Prospective animal study. SETTING: University-based animal research facility.SUBJECTS C57BL/6 mice. INTERVENTIONS: After induction of general anesthesia, cecal ligation and puncture were performed to induce peritonitis and polymicrobial sepsis. The control group had sham laparotomy only. Three samples of spleen and liver were collected from septic and sham animals at 24 hrs after laparotomy. Changes in expression were measured for 588 annotated mouse genes by using a commercially available complementary DNA microarray kit. MEASUREMENTS AND MAIN RESULTS: Broad-scale gene expression profiles were characterized for septic liver and spleen and compared with sham controls. The analytical tools used included commercially available software packages and a novel analysis program. Very little overlap was observed in the septic gene expression profiles of these two organs. Most of the genes identified have previously been linked to regulation of the inflammatory response; importantly, however, some have not. In addition, hierarchical cluster analysis showed that cecal ligation and puncture at 24 hrs induced coordinate expression of genes that alter cell signaling and survival pathways in spleen, consistent with previously published reports of sepsis-induced splenocyte apoptosis. The current limitations of microarray analysis as reflected in these studies are also discussed. CONCLUSIONS: Microarray technology provides a powerful new tool for rapidly analyzing tissue-specific changes in gene expression induced by sepsis in animal models.
Keywords: other
Contributor(s) Cobb JP, Laramie JM, Stormo GD, Morrissey JJ, Shannon WD, Qiu Y, Karl IE, Buchman TG, Hotchkiss RS
Citation(s) 12483063
Submission date Jul 02, 2003
Last update date Feb 23, 2012
Contact name Jason Matthew Laramie
Phone 314-747-0054
Fax 314-362-7474
Organization name Washington University
Department Surgery
Lab Cellular Injury and Adaptation Laboratory
Street address 660 South Euclid Avenue
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL207 Clontech Atlas Mouse cDNA Expression Array (Cat. #7741-1)
Samples (6)
GSM2402 Sham1
GSM2403 Sham2
GSM2404 Sham3
BioProject PRJNA85233

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