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Series GSE51031 Query DataSets for GSE51031
Status Public on Jan 27, 2014
Title DNA methylation status of myelinating Schwann cells during development and in diabetic neuropathy [Bisulfite-Seq2]
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary DNA methylation is a key epigenetic regulator of mammalian embryogenesis and somatic cell differentiation. Using high-resolution genome-scale maps of methylation patterns, we show that the formation of myelin in the peripheral nervous system, proceeds with progressive DNA demethylation, which coincides with an upregulation of critical genes of the myelination process. More importantly, we found that, in addition to expression of DNA methyltransferases and demethylases, the levels of S-adenosylmethionine (SAMe), the principal biological methyl donor, could also play a critical role in regulating DNA methylation during myelination and in the pathogenesis of diabetic neuropathy. In summary, this study provides compelling evidence that SAMe levels need to be tightly controlled to prevent aberrant DNA methylation patterns, and together with recently published studies on the influence of SAMe on histone methylation in cancer and embryonic stem cell differentiation show that in diverse biological processes, the methylome, and consequently gene expression patterns, are critically dependent on levels of SAMe.
 
Overall design DNA methylome maps of mouse Schwann cells in which GNMT was silenced by lentiviral vectors, cultured in normal medium or low methionine medium.
 
Contributor(s) Woodhoo A, Lavin JL
Citation(s) 24607226
Submission date Sep 20, 2013
Last update date May 15, 2019
Contact name Jose Luis Lavin
E-mail(s) joluito@gmail.com
Organization name CIC bioGUNE
Street address Parque Tecnológico de Bizkaia Building 502, Floor 0
City Derio
State/province Bizkaia
ZIP/Postal code 48160
Country Spain
 
Platforms (1)
GPL16173 Illumina HiScanSQ (Mus musculus)
Samples (10)
GSM1235524 SC_Control_1_RRBS
GSM1235525 SC_Control_2_RRBS
GSM1235526 SC_SAMe_1_RRBS
This SubSeries is part of SuperSeries:
GSE45702 DNA methylation status of myelinating Schwann cells during development and in diabetic neuropathy
Relations
BioProject PRJNA219719
SRA SRP030115

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51031_RAW.tar 67.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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