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| Status |
Public on Mar 05, 2014 |
| Title |
A basic helix-loop-helix transcription factors network mediates the brassinosteroid signal [ChIP-seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To investigate the genome-wide occupancies of INCREASED LEAF INCLINATION1 (ILI1)-BINDING bHLH-1 (IBH1) (At2g43060), IBH1 ChIP analysis followed by direct sequencing (ChIP-Seq) on the p35S::IBH1-GFP (IBH1OE) line and a control line p35S::GFP (GFP OE) were conducted. Antibodies against GFP in the GFP-tagged IBH1 were utilized for immunoprecipitation of the endogenous protein-DNA complex, and the obtained DNA was subjected to next generation sequencing.
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| Overall design |
Observation of genome-wide occupancies of atypical bHLH, INCREASED LEAF INCLINATION1 (ILI1)-BINDING bHLH-1 (IBH1) (At2g43060)
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| Contributor(s) |
Zhiponova MK, Morohashi K, Grotewold E, Russinova E |
| Citation(s) |
24505057 |
| |
| Submission date |
Sep 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Kengo Morohashi |
| E-mail(s) |
morohashi.1@osu.edu
|
| Phone |
614-688-4954
|
| Organization name |
The Ohio State University
|
| Department |
Molecular Genetics
|
| Lab |
Erich Grotewold
|
| Street address |
1060 Carmack Rd
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11221 |
Illumina Genome Analyzer IIx (Arabidopsis thaliana) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE51121 |
A basic helix-loop-helix transcription factors network mediates the brassinosteroid signal |
|
| Relations |
| BioProject |
PRJNA221231 |
| SRA |
SRP030400 |
