|
| Status |
Public on Oct 01, 2014 |
| Title |
Identification of MAF controlled genes in breast cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify relevant genes transcriptionally controlled by MAF in breast cancer, we focused on genes whose expression changed accordingly to MAF transcriptiion factor overexpression in MCF7. We used comparative RNA hybridization (Affymetrix) to this end.
|
| |
|
| Overall design |
Total RNA from parental and MAFshort or long isoform overexpressing MCF7 grown for 48 hours in regular media (see growth protocol) was isolated from in vitro cultured control and MAF expressing MCF7 parental cells. Total RNA was extracted using the TRIzol® Plus RNA Purification Kit (Life Technologies).
|
| |
|
| Contributor(s) |
Gomis R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 27, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Evarist Planet Letschert |
| E-mail(s) |
evarist.planet@irbbarcelona.org
|
| Organization name |
IRB Barcelona
|
| Department |
Biostatistics
|
| Street address |
C/ Baldiri Reixac 10
|
| City |
Barcelona |
| State/province |
Spain |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (3) |
|
| This SubSeries is part of SuperSeries: |
| GSE51238 |
16q22-24 DNA amplification specifically predicts and mediates, through MAF gene, breast cancer bone metastasis. |
|
| Relations |
| BioProject |
PRJNA221779 |
