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Status |
Public on Jul 01, 2007 |
Title |
Shigella CGH |
Platform organisms |
Escherichia coli K-12; Shigella flexneri 2a str. 301; Shigella dysenteriae Sd197; Shigella boydii Sb227; Shigella sonnei Ss046 |
Sample organisms |
Escherichia coli; Shigella boydii; Shigella dysenteriae; Shigella flexneri |
Experiment type |
Genome variation profiling by array
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Summary |
Background Compelling evidence indicates that Shigella species, the etiologic agents of bacillary dysentery, as well as enteroinvasive Escherichia coli, are derived from multiple origins of Escherichia coli and form a single pathovar. To further understand the genome diversity and virulence evolution of Shigella, comparative genomic hybridization microarray analysis was employed to compare the gene content of E. coli K-12 with those of 43 Shigella strains from all serotypes.
Results For the 43 strains subjected to CGH microarray analyses, the common backbone of the Shigella genome was estimated to contain more than 1,900 open reading frames, with a mean number of 729 undetectable ORFs. The mosaic distribution of absent regions indicated that insertions and/or deletions have led to the highly diversified genomes of pathogenic strains. Conclusion These results support the hypothesis that by gain and loss of functions, Shigella species became successful human pathogens through convergent evolution from diverse genomic backgrounds. Moreover, we also found many specific differences between different lineages, providing a window into understanding bacterial speciation and taxonomic relationships. Keywords: comparative genomic hybridization
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Overall design |
There are 39 strains used in comparative genomic hybridization, and competitive hybridization was done at least three times for one strain.
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Contributor(s) |
Peng J, Zhang X, Yang J, Wang J, Jin Q |
Citation(s) |
16939645 |
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Submission date |
Jul 02, 2006 |
Last update date |
Mar 16, 2012 |
Contact name |
Junping Peng |
E-mail(s) |
pengjp@gmail.com
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Organization name |
Chinese center for disease control and prevention
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Lab |
State key lab for molecular virology and genetic engineering
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Street address |
No 6, Rongjing Eastern Street, BDA
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City |
Beijing |
ZIP/Postal code |
100176 |
Country |
China |
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Platforms (1) |
GPL3925 |
SLVG E.coli_Shigella 6.5K array |
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Samples (117)
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Relations |
BioProject |
PRJNA96505 |