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Series GSE5212 Query DataSets for GSE5212
Status Public on Jul 01, 2007
Title Shigella CGH
Platform organisms Escherichia coli K-12; Shigella flexneri 2a str. 301; Shigella dysenteriae Sd197; Shigella boydii Sb227; Shigella sonnei Ss046
Sample organisms Escherichia coli; Shigella boydii; Shigella dysenteriae; Shigella flexneri
Experiment type Genome variation profiling by array
Summary Background
Compelling evidence indicates that Shigella species, the etiologic agents of bacillary dysentery, as well as enteroinvasive Escherichia coli, are derived from multiple origins of Escherichia coli and form a single pathovar. To further understand the genome diversity and virulence evolution of Shigella, comparative genomic hybridization microarray analysis was employed to compare the gene content of E. coli K-12 with those of 43 Shigella strains from all serotypes.

Results
For the 43 strains subjected to CGH microarray analyses, the common backbone of the Shigella genome was estimated to contain more than 1,900 open reading frames, with a mean number of 729 undetectable ORFs. The mosaic distribution of absent regions indicated that insertions and/or deletions have led to the highly diversified genomes of pathogenic strains.
Conclusion
These results support the hypothesis that by gain and loss of functions, Shigella species became successful human pathogens through convergent evolution from diverse genomic backgrounds. Moreover, we also found many specific differences between different lineages, providing a window into understanding bacterial speciation and taxonomic relationships.
Keywords: comparative genomic hybridization
 
Overall design There are 39 strains used in comparative genomic hybridization, and competitive hybridization was done at least three times for one strain.
 
Contributor(s) Peng J, Zhang X, Yang J, Wang J, Jin Q
Citation(s) 16939645
Submission date Jul 02, 2006
Last update date Mar 16, 2012
Contact name Junping Peng
E-mail(s) pengjp@gmail.com
Organization name Chinese center for disease control and prevention
Lab State key lab for molecular virology and genetic engineering
Street address No 6, Rongjing Eastern Street, BDA
City Beijing
ZIP/Postal code 100176
Country China
 
Platforms (1)
GPL3925 SLVG E.coli_Shigella 6.5K array
Samples (117)
GSM118082 SB1_1
GSM118083 SB1_2
GSM118084 SB1_3
Relations
BioProject PRJNA96505

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Supplementary data files not provided

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