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| Status |
Public on Apr 24, 2014 |
| Title |
Open lung approach with low tidal volume mechanical ventilation attenuates lung injury in rats with massive brain damage |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Introduction: The ideal ventilation strategy for patients with massive brain damage requires better elucidation. We hypothesized that in the presence of massive brain injury, a ventilation strategy using low (6 mL/kg) tidal volume (VT) ventilation with open lung positive end-expiratory pressure set according to the minimal static elastance of the respiratory system (LVT/OLPEEP), attenuate the impact of massive brain damage on gas-exchange, respiratory mechanics, lung histology and whole genome alterations compared with high (12 mL/kg) VT and low PEEP ventilation (HVT/LPEEP).
Methods: Twenty-eight adult male Wistar rats were randomly assigned to one of four groups: 1) no brain damage (NBD) with LVT/OLPEEP; 2) NBD with HVT/LPEEP; 3) brain damage (BD) with LVT/OLPEEP; and 4) BD with HVT/LPEEP. All animals were mechanically ventilated for six hours. Brain damage was induced by an inflated balloon catheter into the epidural space. Hemodynamics was recorded and blood gas analysis was performed hourly. At the end of the experiment, respiratory system mechanics and lung histology were analysed. Whole genome analysis was performed using Affimetrix gene chips and confirmatory real-time PCR.
Results: In NBD, both LVT/OLPEEP and HVT/LPEEP did not affect arterial blood gases, as well as whole genome expression changes and real-time PCR. In BD, LVT/OLPEEP, compared to HVT/LPEEP, reduced lung damage according to histology, genome analysis and real-time PCR with decreased interleukin (IL-6), cytokine-induced neutrophil chemoattractant (CINC)-1 and angiopoietin-4 expressions. LVT/OLPEEP compared to HVT/LPEEP improved overall survival.
Conclusions: In BD, LVT/OLPEEP minimizes lung morpho-functional changes and inflammation compared to HVT/LPEEP. LVT/OLPEEP might represent a suitable ventilatory strategy in massive brain damage.
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| Overall design |
24 Wistar rats - lung samples, 4 groups,
1. non-braindamaged/braindead high tidal volume ventilation,
2. non-braindamaged/braindead best PEEP ventilation,
3. Braindamaged/braindead high tidal volume ventilation,
4. Braindamaged/braindead best PEEP ventilation
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| Contributor(s) |
Krebs J, Tsagogiorgas C, Pelosi P, Rocco PR, Hottenrott M, Sticht C, Yard B, Luecke T |
| Citation(s) |
24693992 |
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| Submission date |
Nov 06, 2013 |
| Last update date |
Apr 25, 2014 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
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| Department |
ZMF
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| Street address |
Theodor-Kutzer-Ufer
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| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
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| Platforms (1) |
| GPL17896 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] [CDF: Brainarray ENTREZG Version 14] |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA227018 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52142_RAW.tar |
117.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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