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Series GSE52146 Query DataSets for GSE52146
Status Public on Aug 07, 2014
Title High resolution ChIP sequencing reveals novel bindings targets and prognostic role for SOX11 in Mantle cell lymphoma (ChIP-Seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary SOX11 (Sex determining region Y-box 11) expression is specific for MCL as compared to other Non-Hodgkin’s lymphomas. However, the function and direct binding targets of SOX11 in MCL are largely unknown. We used high-resolution ChIP-Seq to identify the direct target genes of SOX11 in a genome-wide, unbiased manner and elucidate its functional significance. Pathway analysis identified WNT, PKA and TGF-beta signaling pathways as significantly enriched by SOX11 target genes. qCHIP confirmed that SOX11 directly binds to individual genes in these pathways in both MCL cell lines and patients. Interrogation of an eighty-two patient  gene-expression dataset demonstrated that SOX11 mRNA expression was inversely proportional to Ki-67, a marker of cell proliferation. Functional studies using RNA interference demonstrate that SOX11 directly regulates WNT signaling and modulates chemotherapy sensitivity to cytarabine in MCL. We analyzed SOX11 expression in three independent well-annotated tissue microarrays from the University of Wisconsin (UW), Karolina Institute and British Columbia Cancer Agency (BCCA). Our findings suggest that high SOX11 expression is associated with improved survival in a subset of MCL patients, particularly those treated with intensive chemotherapy incorporating cytarabine. Transcriptional regulation of WNT and other biological pathways affects by SOX11 target genes may help explain the impact of SOX11 expression on patient outcomes.
Overall design ChIP-seq experiment identifies SOX11 direct binding targets in Granta-519 MCL cell lines
Contributor(s) Parekh S
Citation(s) 24681958
Submission date Nov 06, 2013
Last update date May 15, 2019
Contact name Samir Parekh
Organization name Icahn School of Medicine at Mount Sinai
Department Hematology and Medical Oncology
Street address 1470 Madison Ave, 5th FL, Room 114
City New York
State/province NY
ZIP/Postal code 10029
Country USA
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (4)
GSM1260312 Granta519-SOX11 Run1
GSM1260313 Granta519-SOX11 Run2
GSM1260314 Granta519-IgG Run1
This SubSeries is part of SuperSeries:
GSE52149 High resolution ChIP sequencing reveals novel bindings targets and prognostic role for SOX11 in Mantle cell lymphoma
BioProject PRJNA227024
SRA SRP032752

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Supplementary file Size Download File type/resource
GSE52146_RAW.tar 1.2 Gb (http)(custom) TAR (of BIGBED)
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Raw data are available in SRA
Processed data provided as supplementary file

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