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| Status |
Public on Jan 01, 2010 |
| Title |
Comparison of gene regulation by Gcn5 under KCl stress in S. cerevisiae and S. pombe |
| Organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
This is parallel comparison of gene regulation by Gcn5 in evolutionarily divergent yeasts S. pombe and S. cerevisiae. Our study showed Gcn5 is required for a similar spectrum of stress responses in both organisms, including the response to KCl. This DNA microarray studies is to find conserved or diverged gene regulation pattern under KCl stress condition in the two yeasts. There are 6 subsets in total, 3 subsets in each evolutionarily divergent yeasts including the following: 1 gcn5 mutant compared to wt under rich medium without treatment, 2 gcn5 mutant compared to wild type both under KCl treatment, 3 wild type strains under KCl treatment compared to wild type without treatment.
Keywords: stress response, Gcn5, Comparative genomics, Transcriptional co-activator
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| Overall design |
Gene expression profiling experiment in which gene expression in logarithmically growing cultures of test groups was compared to that of the control groups in rich medium at 25C. There are 6 subsets in total, 3 subsets in each yeast including the following: 1 gcn5 mutant compared to wt under rich medium without treatment, 2 gcn5 mutant compared to wild type both under KCl treatment, 3 wild type strains under KCl treatment compared to wild type without treatment. RNA was extracted and subjected to cDNA expression profiling analysis using the established protocols (Xue et al, 2004). Independent RNA preparations were used to hybridize to microarray slides with dye swap. Data normalized with 'Lowess' per chip per spot normalization.
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| Contributor(s) |
Xue-Franzen Y, Johnsson A, Wright A |
| Citation(s) |
20338033 |
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| Submission date |
Jul 03, 2006 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Anthony Wright |
| E-mail(s) |
yongtao.xue.franzen@ki.se
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| Organization name |
Karolinska Institute
|
| Lab |
Anthony Wright
|
| Street address |
Blickagången 6
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| City |
Huddinge |
| ZIP/Postal code |
141 57 |
| Country |
Sweden |
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| Platforms (4)
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| GPL3424 |
Eurogentec AG S. pombe ORF array 11k (L040B) |
| GPL3425 |
Eurogentec AG S. pombe ORF array 11k (I100C) |
| GPL3426 |
Eurogentec AG S. pombe ORF array 10k (1st batch) |
| GPL3690 |
KI/Eurogentec S. cerevisiae 12.8K array (E150C) |
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| Samples (18)
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| GSM106754 |
Saccharomyces cerevisiae wild type KCl treatment replicate 2 |
| GSM106826 |
Saccharomyces cerevisiae wild type KCl treatment replicate 1 |
| GSM106829 |
Saccharomyces cerevisiae gcn5 mutant KCl treatment replicate 1 |
| GSM106830 |
Saccharomyces cerevisiae gcn5 mutant KCl treatment replicate 2 |
| GSM106831 |
Saccharomyces cerevisiae gcn5 mutant replicate 1 |
| GSM106832 |
Saccharomyces cerevisiae gcn5 mutant replicate 2 |
| GSM107677 |
Schizosaccharomyces Pombe wild type strain KCl treatment, replicate 3 |
| GSM107679 |
Schizosaccharomyces Pombe wild type strain KCl treatment, replicate 4 |
| GSM116708 |
Schizosaccharomyces Pombe wild type strain KCl treatment, replicate 1 (slide 19) |
| GSM117357 |
Schizosaccharomyces Pombe wild type strain KCl treatment, replicate 2 (slide 17) |
| GSM118250 |
Schizosaccharomyces Pombe gcn5 mutant compared to wt no treatment,replicate 1 (slide 13) |
| GSM118251 |
Schizosaccharomyces Pombe gcn5 mutant compared to wt no treatment,replicate 2 (slide 9) |
| GSM118252 |
Schizosaccharomyces pombe gcn5 mutant compared to wt no treatment,replicate 3 (slide 18) |
| GSM118253 |
Schizosaccharomyces Pombe gcn5 mutant compared to wt no treatment,replicate 4 (slide 21) |
| GSM118254 |
Schizosaccharomyces Pombe gcn5 mutant compared to wt no treatment,replicate 5 (slide 22) |
| GSM118255 |
Schizosaccharomyces Pombe gcn5 mutant compared to wt no treatment,replicate 6 (slide 23) |
| GSM118256 |
Schizosaccharomyces Pombe gcn5 mutant compared to wt, KCl treatment,replicate 1 (slide 5) |
| GSM118257 |
Schizosaccharomyces pombe gcn5 mutant compared to wt KCl treatment,replicate 1 (slide 6) |
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| This SubSeries is part of SuperSeries: |
| GSE16556 |
Role of Gcn5 in KCl stress adaptation in S. cerevisiae and S. pombe: transcriptional and genomic analyses |
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| Relations |
| BioProject |
PRJNA122641 |
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