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Series GSE5227 Query DataSets for GSE5227
Status Public on Jul 06, 2006
Title Stress-specific role of the fission yeast Gcn5 acetylatransferase in programming a subset of stress-response genes
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by array
Summary We have found Gcn5 is specifically requried for KCl and CaCl2 mediated stress in Schizosaccharomyces Pombe. We have charaterised the genome wide gene expression responses to KCl stress and show the Gcn5 in involved in the regulation of sebset of stress response genes.
Keywords: stress response
Overall design Four subsets is included: 1 gcn5 mutant compared to wt under rich medium without treatement, 2 gcn5 mutant compared to wild type both under KCl treatment, 3 wild type 15min treatment with KCl compared to non-treated, 4 Wild type 60min KCl treatement compared to non-treated. Gene expression profiling experiment in which gene expression in logarithmically growing cultures of test group was compared to that control in rich medium at 25C.RNA was extracted and subjected to cDNA expression profiling analysis using the established protocols (Xue et al, 2004). Independent RNA preparations were used to hybridise to microarray slides with dye swap. Data normalized with 'Lowess' per chip per spot normalization.
Contributor(s) Johnsson A, Xue-Franzen Y, Wright A
Citation(s) 16896217
Submission date Jul 04, 2006
Last update date Mar 16, 2012
Contact name Anthony Wright
Organization name Karolinska Institute
Lab Anthony Wright
Street address Blickagången 6
City Huddinge
ZIP/Postal code 141 57
Country Sweden
Platforms (3)
GPL3424 Eurogentec AG S. pombe ORF array 11k (L040B)
GPL3425 Eurogentec AG S. pombe ORF array 11k (I100C)
GPL3426 Eurogentec AG S. pombe ORF array 10k (1st batch)
Samples (12)
GSM116708 Schizosaccharomyces Pombe wild type strain KCl treatment, replicate 1 (slide 19)
GSM117357 Schizosaccharomyces Pombe wild type strain KCl treatment, replicate 2 (slide 17)
GSM117358 Schizosaccharomyces Pombe wild type strain KCl treatment 15min, replicate 1 (slide16)
BioProject PRJNA96427

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