|
Status |
Public on Jul 05, 2006 |
Title |
Expression data from Lbeta T2 cells upon activin treatment |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
The primary response of Lbeta T 2cells upon activin treatment at 1hr, 2hr, and 6hr is the significant up-regulation of FSHbeta subunit. Affymetrix genearray missed this important information due to cross-hybridization of its probes with other genes and chromosome segments. Activin modulates a network of genes and signaling pathways to exert a variety of functions. Keywords: time course
|
|
|
Overall design |
Subconfluent Lbeta T2 cells were seeded in cell culture flasks for 16 hours in phenol-free, serum-free medium, and then treated with either vehicle or activin A (30ng/ml). At 1hr, 2hr, or 6hr after treatment, total RNA was isolated using RNeasy Mini Kit. Integrity of RNA was evaluated with an Agilent 2100 Bioanalyzer, and purity/concentration was determined using a GeneSpec III before target preparationi and hybridizations.
|
|
|
Contributor(s) |
Peng Y, Li X, Woodruff T |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Jul 05, 2006 |
Last update date |
Feb 11, 2019 |
Contact name |
Ying Peng |
E-mail(s) |
yingp@northwestern.edu
|
Organization name |
Northwestern University
|
Street address |
2205 Tech Drive
|
City |
Evanston |
State/province |
IL |
ZIP/Postal code |
60208 |
Country |
USA |
|
|
Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA96437 |