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Series GSE5232 Query DataSets for GSE5232
Status Public on Jul 05, 2006
Title Expression data from Lbeta T2 cells upon activin treatment
Organism Mus musculus
Experiment type Expression profiling by array
Summary The primary response of Lbeta T 2cells upon activin treatment at 1hr, 2hr, and 6hr is the significant up-regulation of FSHbeta subunit. Affymetrix genearray missed this important information due to cross-hybridization of its probes with other genes and chromosome segments.
Activin modulates a network of genes and signaling pathways to exert a variety of functions.
Keywords: time course
Overall design Subconfluent Lbeta T2 cells were seeded in cell culture flasks for 16 hours in phenol-free, serum-free medium, and then treated with either vehicle or activin A (30ng/ml). At 1hr, 2hr, or 6hr after treatment, total RNA was isolated using RNeasy Mini Kit. Integrity of RNA was evaluated with an Agilent 2100 Bioanalyzer, and purity/concentration was determined using a GeneSpec III before target preparationi and hybridizations.
Contributor(s) Peng Y, Li X, Woodruff T
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Submission date Jul 05, 2006
Last update date Feb 11, 2019
Contact name Ying Peng
Organization name Northwestern University
Street address 2205 Tech Drive
City Evanston
State/province IL
ZIP/Postal code 60208
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM118434 Lbeta T2_1hr Activin
GSM118435 Lbeta T2_1hr Control
GSM118436 Lbeta T2_2hr Activin
BioProject PRJNA96437

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5232_1hr-Activin_vs_1hr-Cntl_1.5-fold.xls 41.5 Kb (ftp)(http) XLS
GSE5232_2hr-Activin_vs_2hr-Cntl_1.5-fold.xls 41.5 Kb (ftp)(http) XLS
GSE5232_6hr-Activin_vs_6hr-Cntl_1.5-fold.xls 41.5 Kb (ftp)(http) XLS
GSE5232_Activin_vs_Cntl_All3timepts_1.2-fold.xls 41.5 Kb (ftp)(http) XLS
GSE5232_Signaling-pathways_Activin_3timepts.xls 41.5 Kb (ftp)(http) XLS

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