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Series GSE52347 Query DataSets for GSE52347
Status Public on Apr 15, 2014
Title The nucleosomal barrier to promoter escape by RNA Polymerase II is overcome by the chromatin remodeler Chd1 [NimbleGen]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary We use a metabolic labeling strategy for directly measuring nucleosome turnover to determine the role of chromatin remodeler Chd1 on chromatin dynamics in mouse embryonic fibroblasts expressing with wildtype-Chd1 and dominant negative K510R-Chd1. We find that expression of K510R-Chd1 decreases nucleosome turnover at the promoter and increases turnover within the gene body.
 
Overall design 10 Illumina sequencing samples and 4 Nimblegen array samples
 
Contributor(s) Skene PJ, Hernandez AE, Groudine M, Henikoff S
Citation(s) 24737864
Submission date Nov 13, 2013
Last update date Apr 25, 2014
Contact name Jorja Henikoff
E-mail(s) jorja@fhcrc.org
Phone 206-667-4850
Organization name Fred Hutchinson Cancer Research Center
Department Basic Sciences
Lab Henikoff
Street address 1100 Fairview AV N, A1-162
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platforms (1)
GPL16533 NimbleGen Mouse 2.1M tiling (MM9_Deluxe_Prom_ChIP_HX1)
Samples (4)
GSM1263785 K510R_MEFs_CATCHIT_(20121012_538427_2)
GSM1263786 KD_MEFs_CATCHIT_(20121012_559018)
GSM1263787 control_MEFs_CATCHIT_(20131025_559020)
This SubSeries is part of SuperSeries:
GSE52349 The nucleosomal barrier to promoter escape by RNA Polymerase II is overcome by the chromatin remodeler Chd1
Relations
BioProject PRJNA229135

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52347_RAW.tar 703.0 Mb (http)(custom) TAR (of GFF, PAIR, WIG)
Processed data included within Sample table
Processed data provided as supplementary file

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