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| Status |
Public on Jan 08, 2014 |
| Title |
Expression analysis of Caulobacter crescentus NA1000 delta-CCNA_00382 (ccrM) [DccrM] vs Caulobacter crescentus NA1000 wild type [WT] |
| Organism |
Caulobacter vibrioides NA1000 |
| Experiment type |
Expression profiling by array
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| Summary |
Investigation of whole genome gene expression level changes in a Caulobacter crescentus NA1000 delta-CCNA_00382 (ccrM) mutant, compared to the wild-type strain. The mutations engineered into this strain render it incapable of methylating its genome on the adenine at GANTC motifs.
References for strains : WT: Marks, M.E., Castro-Rojas, C.M., Teiling, C., Du, L., Kapatral, V., Walunas, T.L. and Crosson, S. (2010) The genetic basis of laboratory adaptation in Caulobacter crescentus. J Bacteriol, 192, 3678-3688; Collier, J. and Shapiro, L. (2009) Feedback control of DnaA-mediated replication initiation by replisome-associated HdaA protein in Caulobacter. J Bacteriol, 191, 5706-5716. DccrM: Gonzalez, D. and Collier, J. (2013) DNA methylation by CcrM activates the transcription of two genes required for the division of Caulobacter crescentus. Mol Microbiol, 88, 203-218.
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| Overall design |
A six chip study using total RNA recovered from three separate wild-type cultures of Caulobacter crescentus NA1000 and three separate cultures of a triple mutant strain, Caulobacter crescentus NA1000 delta-CCNA_00382 (ccrM), in which the ccrM gene coding for a DNA methyltransferase methylating the adenine in GANTC motifs is truncated and its product inactive. Each chip measures the expression level of 3933 genes from Caulobacter crescentus NA1000 with 3 probes per gene and with three-fold technical redundancy.
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| Contributor(s) |
Diego G, Justine C |
| Citation(s) |
24398711 |
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| Submission date |
Nov 14, 2013 |
| Last update date |
Feb 06, 2014 |
| Contact name |
Diego Gonzalez |
| Organization name |
University of Lausanne
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| Department |
Department of Fundamental Microbiology
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| Lab |
Prof. Justine Collier
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| Street address |
Quartier UNIL-Sorge, Bâtiment Biophore
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| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL17931 |
110420_Ccre_JC_exp, 4plex array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA227736 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52375_RAW.tar |
10.8 Mb |
(http)(custom) |
TAR (of PAIR) |
| Processed data included within Sample table |
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