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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 18, 2013 |
| Title |
Expression data from mouse liver over-expressing either GFP alone or biotinylated-TRb1 and GFP |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We over-expressed biotinylated-thyroid hormone receptor beta 1 (TRb1) in mouse liver using an adenovirus in order to perform ChIP-seq experiments. These microarrays were performed to determine gene expression changes in response to tri-iodothyronine (thyroid hormone; T3) stimulation. A control GFP adenovirus was used and gene expression from these livers was also done as a comparison.
We performed microarrays from Ad-GFP-infected propylthiouracil (PTU)-fed livers injected with either saline or T3 and Ad-TRb1-GFP infected livers injected with either saline or T3.
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| Overall design |
RNA was extracted from livers of biotin ligase (BirA)-expressing mice that had been infected with either Ad-GFP or Ad-TRb1, fed with PTU for 3 weeks followed by saline or T3 injections for 4 consecutive days.
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| Contributor(s) |
Ramadoss P, Abraham BJ, Tsai L, Zhou Y, Costa-e-Sousa RH, Ye F, Bilban M, Zhao K, Hollenberg AN |
| Citation(s) |
24288132 |
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| Submission date |
Nov 15, 2013 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Preeti Ramados |
| Organization name |
Beth Israel Deaconess Medical Center
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| Street address |
3 Blackfan Cir
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| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM1266759 |
Ad-GFP infected liver, PTU fed and saline treated, biological replicate 1 |
| GSM1266760 |
Ad-GFP infected liver, PTU fed and saline treated, biological replicate 2 |
| GSM1266761 |
Ad-GFP infected liver, PTU fed and saline treated, biological replicate 3 |
| GSM1266762 |
Ad-GFP infected liver, PTU fed and T3 treated, biological replicate 1 |
| GSM1266763 |
Ad-GFP infected liver, PTU fed and T3 treated, biological replicate 2 |
| GSM1266764 |
Ad-GFP infected liver, PTU fed and T3 treated, biological replicate 3 |
| GSM1266765 |
Ad-TRb infected liver, PTU fed and saline treated, biological replicate 1 |
| GSM1266766 |
Ad-TRb infected liver, PTU fed and saline treated, biological replicate 2 |
| GSM1266767 |
Ad-TRb infected liver, PTU fed and saline treated, biological replicate 3 |
| GSM1266768 |
Ad-TRb infected liver, PTU fed and T3 treated, biological replicate 1 |
| GSM1266769 |
Ad-TRb infected liver, PTU fed and T3 treated, biological replicate 2 |
| GSM1266770 |
Ad-TRb infected liver, PTU fed and T3 treated, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA228921 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52433_RAW.tar |
117.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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