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| Status |
Public on Dec 26, 2013 |
| Title |
Effects of RNAi protein overexpression in mutant cells on H3K9 dimethylation in fission yeast cells |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We test the effects of overexpressing RNAi proteins Dcr1, Rdp1, or Ago1 in wildtype, dcr1∆, ago1∆, or rdp1∆ cells. We find that overexpression does not generally affect H3K9me2 at euchromatic loci, but there are varying effects in H3K9me2 on centromeric heterochromatin.
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| |
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| Overall design |
Examination of genome-wide enrichment in H3K9 dimethylation in fission yeast haploid cells
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| |
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| Contributor(s) |
Yu R, Jih G, Moazed D |
| Citation(s) |
24374313 |
| |
| Submission date |
Nov 18, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Ruby Yu |
| Organization name |
Harvard University
|
| Department |
Cell Biology
|
| Lab |
Danesh Moazed
|
| Street address |
240 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13988 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe) |
|
| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE52535 |
Determinants of heterochromatic siRNA biogenesis and function |
|
| Relations |
| BioProject |
PRJNA228964 |
| SRA |
SRP033087 |
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