|
| Status |
Public on Dec 26, 2013 |
| Title |
Role of 3' UTR in spreading of siRNAs |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We use small RNA sequencing to look for spreading of secondary and tertiary siRNAs along transcripts targeted by ectopic hairpin-derived primary siRNAs. We find that signals within the 3' UTR inhibit siRNA spreading.
|
| |
|
| Overall design |
sequencing of 20-30nt sRNAs from Argonaute immunoprecipitation
|
| |
|
| Contributor(s) |
Yu R, Moazed D |
| Citation(s) |
24374313 |
| |
| Submission date |
Nov 19, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Ruby Yu |
| Organization name |
Harvard University
|
| Department |
Cell Biology
|
| Lab |
Danesh Moazed
|
| Street address |
240 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15167 |
Illumina Genome Analyzer IIx (Schizosaccharomyces pombe) |
|
| Samples (7)
|
|
| This SubSeries is part of SuperSeries: |
| GSE52535 |
Determinants of heterochromatic siRNA biogenesis and function |
|
| Relations |
| BioProject |
PRJNA229168 |
| SRA |
SRP033134 |
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