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| Status |
Public on Mar 04, 2014 |
| Title |
A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Expression data from CD34+ hematopoietic cells transduced with control or anti-SLPI shRNA, serum starved and treated with G-CSF.
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| |
|
| Overall design |
CD34+ hematopoietic cells were isolated from the bone marrow of healthy individuals, transduced with lenitvirus-based RFP-expressed shRNA constructs targeting SLPI mRNA or scrambled control shRNA. After 3 days of the transductions, RFP+ cells were sorted, serum starved for 24 hours and treated with G-CSF 10 ng/ml for 18 hours. Total RNA of sorted cells was isolated and used for Affymetrix Exome arrays.
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| |
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| Contributor(s) |
Skokowa J, Klimenkova O |
| Citation(s) |
24352879 |
| |
| Submission date |
Dec 16, 2013 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Siarhei Kandabarau |
| E-mail(s) |
kandabarau.siarhei@mh-hannover.de
|
| Organization name |
Hannover Medical School
|
| Street address |
Carl-Neuberg-Str 1
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA231777 |
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