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Series GSE53421 Query DataSets for GSE53421
Status Public on Dec 18, 2013
Title Long term demasculinization of X-linked intergenic noncoding RNAs in Drosophila melanogaster
Organism Drosophila melanogaster
Experiment type Genome variation profiling by genome tiling array
Summary Recent studies have revealed key roles of non-coding RNAs in sex-related pathways, but little is known about the evolutionary forces acting on these non-coding RNAs. We used whole-genome tiling arrays to profile the transcriptome of Drosophila melanogaster tissues and found that 15% of male-biased transcribed fragments (transfrags) are intergenic non-coding RNAs (incRNAs), suggesting a potentially important role for incRNAs in sex-related biological processes. Statistical analysis revealed a paucity of male-biased incRNAs and coding genes on the X chromosome, suggesting that similar evolutionary forces could be affecting the genomic organization of both coding and non-coding genes. Expression profiling across germline and somatic tissues further suggests that both male meiotic sex chromosome inactivation (MSCI) and sexual antagonism contribute to the chromosomal distribution of male-biased incRNAs. Comparative sequence analysis shows that the evolutionary age of male-biased incRNAs is a significant predictor of their chromosomal locations. In addition to identifying abundant sex-biased incRNAs in fly genome, our work unveils a global picture of the complex interplay between non-coding RNAs and sexual chromosome evolution.
Overall design We used whole-genome tiling arrays to assess sex-biased transcription in D. melanogaster adult whole bodies, and testis, ovary, and accessory gland samples. Gut and thorax, tissues expected to exhibit little or no sex-biased expression, were included as controls. Expression was measured in 2-4 replicates for each tissue.
Contributor(s) Gao G, Vibranovski MD, Zhang L, Li Z, Liu M, Zhang YE, Li X, Zhang W, Fan Q, VanKuren NW, Long M, Wei L
Citation(s) 24407956
Submission date Dec 17, 2013
Last update date Sep 09, 2014
Contact name Maria D Vibranovski
Organization name Universidade de Sao Paolo
Department Genetics and Evolutionary Biology
Street address Rua do Matao, 277
City Sao Paulo
ZIP/Postal code 05508-090
Country Brazil
Platforms (1)
GPL11397 [Dm35b_MF] Affymetrix Drosophila Tiling 1.0F Array
Samples (9)
GSM1291177 Accessory gland
GSM1291178 Female whole body
GSM1291179 Female gut
BioProject PRJNA231948

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53421_RAW.tar 859.5 Mb (http)(custom) TAR (of BAR, CEL)
Processed data provided as supplementary file

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