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| Status |
Public on Jan 30, 2014 |
| Title |
Deciphering life history transcriptomics in different environments |
| Organism |
Drosophila mojavensis |
| Experiment type |
Expression profiling by array
|
| Summary |
Investigation of whole genome transcription expression level changes in Drosophila mojavensis wild-type populations (Las Bocas:LB and Punta Prieta:PP). The experiment was designed to investigate life history transcriptomics in different environments.
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| Overall design |
A total of 172 hybridizations were performed in this entire experiment. We used 135K 12-plex NimbleGen arrays. Total RNA was recovered from each sample listed below. The experimental design consisted a total of two populations (Las Bocas:LB; Punta Prieta:PP), two host diets (Agria:AG and Organ pipe:OP) and fourteen life stages (6 hr egg:E6; 1st instar larvae:L1; 2nd instar larvae:L2; 3rd instar larvae:L3; Early pupal stage:EP; Late pupal stage:LP; 0 Day old adult:0D; 3 Day old adult:3D; 4 Day old adult: 4D; 6 Day old adult:6D; 10 Day old adult:10D; 14 Day old adult:14D; 18 Day old adult:18D & 24 Day old adult:24D). Each chip measures the expression level of 14528 transcripts. Two to 5 replicates were used for each type (R1, R2, R3 etc.). Fly source details are as follows: Las Bocas 2009: LB09; Punta Prieta 2008:PP08.
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| Contributor(s) |
Gibbs AG, Etges WJ, Tuljapurkar S, Trotter MV, de Oliveira CC, Rajpurohit S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 29, 2014 |
| Last update date |
Jan 30, 2014 |
| Contact name |
Allen G. Gibbs |
| E-mail(s) |
allen.gibbs@unlv.edu
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| Phone |
702-895-3203
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| Organization name |
University of Nevada
|
| Department |
School of Life Sciences
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| Lab |
Gibbs Lab
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| Street address |
4505 S Maryland Parkway
|
| City |
Las Vegas |
| State/province |
Nevada |
| ZIP/Postal code |
89154 |
| Country |
USA |
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| Platforms (1) |
| GPL16433 |
NimbleGen Gibbs Drosophila mojavensis 135K 12-plex Array |
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| Samples (172)
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| Relations |
| BioProject |
PRJNA236745 |
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