|
| Status |
Public on Mar 17, 2014 |
| Title |
Rbfox2 controls autoregulation in RNA binding protein networks |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
We generated a global analysis of Rbfox2 splicing regulation combined with a highly specific, single nucleotide-resolution Rbfox2 RNA binding map. We found that Rbfox2 regulates the splicing and expression of many previously unknown targets, and particularly a number of RNA binding proteins (RBPs), by modulating alternative splicing coupled-NMD. Based on our observations of RBP-Rbfox2 co-regulation with a polarity predicted by Rbfox2 binding, we propose a model whereby Rbfox2 tunes autoregulatory splicing events to control RBP expression levels and in turn alter their respective splicing networks.
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| |
|
| Overall design |
iCLIP for epitope-tagged Rbfox2 and control untagged Rbfox2; RNAseq of control and Rbfox2 knockdown in mouse embryonic stem cells
|
| |
|
| Contributor(s) |
Jangi M, Sharp PA |
| Citation(s) |
24637117 |
| |
| Submission date |
Feb 07, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Mohini Jangi |
| Organization name |
MIT
|
| Department |
Biology
|
| Lab |
Sharp
|
| Street address |
500 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA237658 |
| SRA |
SRP036863 |
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