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Series GSE54811 Query DataSets for GSE54811
Status Public on Mar 01, 2014
Title Bone marrow macrophage (BMMF) response to IFNgR+FcgRI coincidence detection
Organism Mus musculus
Experiment type Expression profiling by array
Summary Different cell types that make an organism use a small number of identical signaling modules to integrate external cues and elicit diverse functions. How the specificity of functional outcomes is achieved is unclear. It must lie within the context in which the external cue is perceived and integrated by the target cell. Within the innate immune system, we discovered communication between two classes of heterologous receptors designed to integrate external cues into context-specific response. Using IFNgR as an example, we describe the structural and functional collaboration between cytokine receptors and the ITAM signaling module, in this case the Fcg-chain at the plasma membrane of phagocytes. Receptor coupling allowed IFNg to modify FcgRI responses during IgG-mediated phagocytosis and to specify cell autonomous antimicrobial functions.
Overall design BMMF were left unstimulated or were stimulated in duplicates for 6 hrs with IFN╬│, IgG2a-opsonised SRBC or both. cDNA was analyzed using 8-sample Mouse Ref-8 v2.0 Illumina array. Expressed genes were defined as those with p<0.05, and their relative expression values were calculated using unstimulated cells as calibrator. IgG2a against SRBC was produced by hybridoma TIB111 from ATCC
Contributor(s) Ruslan M
Citation(s) 24608040
Submission date Feb 10, 2014
Last update date Jun 14, 2018
Contact name Ruslan Medzhitov
Organization name Yale University
Department Immunobiology
Street address PO Box 208011 300 Cedar Street TAC S660
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM1324353 Non stim 1
GSM1324354 SRBC-IgG2a ops 1
GSM1324355 Non stim 2
This SubSeries is part of SuperSeries:
GSE54824 A role for the ITAM signaling module in specifying cytokine receptor functions
BioProject PRJNA237761

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54811_RAW.tar 3.1 Mb (http)(custom) TAR
GSE54811_non-normalized.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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