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| Status |
Public on Feb 01, 2019 |
| Title |
Aryl hydrocarbon receptor antagonism allows sustained culture of leukemia progenitor cells from acute myeloid leukemia patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Hematopoietic progenitors from AML patients express the aryl hydrocarbon receptor signaling pathway. Independent of in vivo engraftability to detect leukemic stem cells, AHR antagonism allows in vitro survival and expansion of AML progenitors, while retaining patient-to-patient heterogeneity.
Based on AML expression data, we show that AHR antagonism disrupts the AHR signaling pathway but it doesn't affect the overall genomic profile of the samples
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| Overall design |
Primary human mononuclear (Ficoll-separated) AML cells were treated with DMSO vehicle control or purine derivative and AHR antagonist (PD-AHA) analog to stemregenin 1 for 24 h in a serum-free media supplemented with stem cell factor, FLT3 ligand and thrombopoietin
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| Contributor(s) |
Casado FL, Guezguez B, McNicol JD, Fiebig-Comyn A, Shapovalova Z, Salci K, Collins TJ, Bhatia M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 21, 2014 |
| Last update date |
Feb 02, 2019 |
| Contact name |
Mickie Bhatia |
| E-mail(s) |
mbhatia@mcmaster.ca
|
| Organization name |
McMaster University
|
| Department |
Stem Cell and Cancer Research Institute (SCC-RI)
|
| Street address |
1200 Main Street West
|
| City |
Hamilton |
| State/province |
Ontario |
| ZIP/Postal code |
L8N 3Z5 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (14)
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| GSM1332310 |
mononuclear blood cell_DMSO_AML14170_24h |
| GSM1332311 |
mononuclear blood cell_DMSO_AML14362_24h |
| GSM1332312 |
mononuclear blood cell_DMSO_AML14366_24h |
| GSM1332313 |
mononuclear blood cell_DMSO_AML15331_24h |
| GSM1332314 |
mononuclear blood cell_DMSO_AML16626_24h |
| GSM1332315 |
mononuclear blood cell_DMSO_AML16626_1_24h |
| GSM1332316 |
mononuclear blood cell_DMSO_AML18947_24h |
| GSM1332317 |
mononuclear blood cell_AHR antagonist_AML14170_24h |
| GSM1332318 |
mononuclear blood cell_AHR antagonist_AML14362_24h |
| GSM1332319 |
mononuclear blood cell_AHR antagonist_AML14366_24h |
| GSM1332320 |
mononuclear blood cell_AHR antagonist_AML15331_24h |
| GSM1332321 |
mononuclear blood cell_AHR antagonist_AML16626_24h |
| GSM1332322 |
mononuclear blood cell_AHR antagonist_AML16626_1_24h |
| GSM1332323 |
mononuclear blood cell_AHR antagonist_AML18947_24h |
|
| Relations |
| BioProject |
PRJNA238985 |
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