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| Status |
Public on Jun 03, 2014 |
| Title |
High-throughput SuperSAGE for transcript profiling in berry tissues of Vitis vinifera L. under different light regimes |
| Organism |
Vitis vinifera |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
SuperSAGE is a method of digital gene expression profiling that allows isolation of 26bp tag fragments from expressed transcripts. Because its tag size is larger than that of conventional SAGE, SuperSAGE allowed a secure tag-to-gene annotation using BLAST search against grape genome databases.Transcript profiles in nine samples of grape berry tissues under different light conditions were obtained by SuperSAGE analysis and used for screening the genes which have co-ordinated transcript profiles with the change in the flavonoid composition in the samples analyzed. Candidate genes related to flavonoid biosynthesis and regulation were identified.
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| Overall design |
Nine different grape samples, i.e., flowers, grape berries of Cabernet Sauvignon at 2, 7, 9 weeks after flowering (WAF), berry skins at 17 days after flowering (DAF) shaded after flowering, and berry skins at 17DAF shaded from flowering to 14DAF and then light exposed, were analyzed.
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| Contributor(s) |
Koyama K, Matsumura H |
| Citation(s) |
24860184 |
| |
| Submission date |
Feb 28, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Kazuya Koyama |
| E-mail(s) |
koyama@nrib.go.jp
|
| Phone |
+81-82-420-0812
|
| Organization name |
National Research Institute of Brewing
|
| Department |
Fundamental Reesearch Division
|
| Street address |
3-7-1, Kagamiyama
|
| City |
Higashi-Hiroshima |
| State/province |
Hiroshima |
| ZIP/Postal code |
739-0046 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL14639 |
Illumina Genome Analyzer II (Vitis vinifera) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE55474 |
Functional characterization of a new grapevine MYB transcription factor and regulation of proanthocyanidin biosynthesis in grapes |
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| Relations |
| BioProject |
PRJNA239708 |
| SRA |
SRP039083 |
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